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COVID-19: Viral Transport Medium (VTM) for Diagnosis

The success of diagnosis of SARS-CoV-2 (2019-nCoV) during the COVID-19 outbreak largely depends on the quality of the sample and the conditions under which the sample is transported and stored before being processed in the laboratory. Viral transport medium (VTM) allows the safe transfer of viruses, chlamydia and mycoplasma for further research, which includes conventional …

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Immunohistochemical and clinical characterization of the basal-like subtype of invasive breast carcinoma

Immunohistochemical and medical characterization of the basal-like subtype of invasive breast carcinoma.   OBJECTIVE Expression profiling research categorized breast carcinomas into estrogen receptor (ER)+/luminal, regular breast-like, HER2 overexpressing, and basal-like teams, with the latter two related to poor outcomes. At the moment, there exist medical assays that determine ER+/luminal and HER2-overexpressing tumors, and we sought to develop …

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Genome-wide DNA methylation profiling using Infinium assay

Genome-wide DNA methylation profiling utilizing Infinium® assay.   OBJECTIVE Bisulfite sequence evaluation of particular person CpG websites inside genomic DNA is a robust method for methylation evaluation within the genome. The main limitation of bisulfite-based strategies is parallelization. Each array and next-generation sequencing know-how are succesful of addressing this bottleneck. On this report, we describe …

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Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment

Heterogeneity and hierarchy inside probably the most primitive hematopoietic stem cell compartment. Hematopoietic stem cells (HSCs) have been extensively characterised primarily based on practical definitions decided by experimental transplantation into lethally irradiated mice. In mice, HSCs are heterogeneous with regard to self-renewal potential, in vitro colony-forming exercise, and in vivo conduct. We tried potential isolation …

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