HRP Conjugated Arachis hypogaea
Software program Widespread Western Blot Protocol: Glycoprotein sample dimension: 500ng Lectin Focus: 0.1ug/ml Load samples at 500 ng of glycoprotein per lane Run 4-20% Bis-Tris SDS net web page gel Swap gel to a PVDF membrane Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma) Incubate HRP lectin at 0.1ug/ml with RIPA …