Software program
Widespread Western Blot Protocol:
- Glycoprotein sample dimension: 500ng
- Lectin Focus: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS net web page gel
- Swap gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Arachis hypogaea (Peanut) Lectin (PNA) – HRP
Abstract
Peanut (Arachis hypogaea) agglutinin (PNA) is extensively used as tumour arker it strongly recognises probably the most cancers specific T antigen (Galbeta1–>3GalNAc-), nonetheless not its sialylated mannequin. Nonetheless, an additional specificity within the path of Galbeta1–>4GlcNAc (LacNAc), which is not tumour specific, had been attributed to PNA.
For correct interpretation of lectin histochemical outcomes we examined PNA sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, moderately than mono- or disaccharides as ligands. Dot-blots, swap blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and totally different lectins of acknowledged specificity.
Modifications of PNA-binding glycoproteins, along with selective eradicating of O-linked oligosaccharides and remedy with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) along with uncovered T antigen (Galbeta1–>Three GalNAc-) was great as sugar moiety in glycoproteins for his or her recognition by PNA. When immobilised, melibiose was superior to lactose in PNA binding. Outcomes have been confirmed using TAG-specific human serum anti-alpha-galactoside antibody.
![]() Arachis hypogaea lectin (PNA) |
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05-0116-10 | Medicago | 10 mg | EUR 116 |
![]() Arachis hypogaea lectin (PNA |
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05-0116-1000 | Medicago | 1 g | Ask for price |
![]() Arachis hypogaea lectin (PNA |
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05-0116-50 | Medicago | 50 mg | EUR 314 |
![]() Arachis hypogaea Arachin Ahy-3 |
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1-CSB-EP737391ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Arachin Ahy-3 expressed in E.coli |
![]() Arachis hypogaea Arachin Ahy-3 |
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1-CSB-YP737391ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Arachin Ahy-3,partial expressed in Yeast |
![]() Arachis hypogaea Allergen Ara h 1, clone P17 |
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1-CSB-YP331779ANE(A4) | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in Yeast |
![]() Arachis hypogaea Allergen Ara h 1, clone P17 |
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1-CSB-EP331779ANE(A4) | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in E.coli |
![]() Arachis hypogaea Allergen Ara h 1, clone P17 |
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1-CSB-EP331779ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17,partial expressed in E.coli |
![]() Arachis hypogaea Allergen Ara h 1, clone P41B |
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1-CSB-CF337356ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P41B expressed in in vitro E.coli expression system |
![]() Biotin [HRP] |
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DAG3197-HRP | Creative Diagnostics | 1mg | EUR 429 |
![]() GAPDH Antibody - HRP |
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T0004-HRP | Affbiotech | 1ml | EUR 920 |
Description: GAPDH Mouse Monoclonal antibody detects endogenous levels of total GAPDH protein. |
![]() Vitamine B9 [HRP] |
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DAGDT1440-HRP | Creative Diagnostics | 1mg | EUR 1105 |
![]() Vitamine B12 [HRP] |
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DAGT5413-HRP | Creative Diagnostics | 1mg | EUR 975 |
![]() CF488A Arachis hypogaes Lectin PNA (Peanut) |
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29060 | Biotium | 1mg | EUR 195 |
Description: Minimum order quantity: 1 unit of 1mg |
![]() CF568 Arachis hypogaes Lectin PNA (Peanut) |
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29061 | Biotium | 1mg | EUR 195 |
Description: Minimum order quantity: 1 unit of 1mg |
![]() CF594 Arachis hypogaes Lectin PNA (Peanut) |
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29062 | Biotium | 1mg | EUR 195 |
Description: Minimum order quantity: 1 unit of 1mg |