HRP Conjugated Arachis hypogaea

Software program

Widespread Western Blot Protocol:

Glycoprotein sample dimension: 500ng
Lectin Focus: 0.1ug/ml

Load samples at 500 ng of glycoprotein per lane
Run 4-20% Bis-Tris SDS net web page gel
Swap gel to a PVDF membrane
Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
Wash membrane 5 x 5 minutes with 25ml RIPA buffer
Detect using chemiluminescent substrate (CPS1-120)

Arachis hypogaea (Peanut) Lectin (PNA) – HRP

Affinity-purified PNA is conjugated to horseradish peroxidase (HRP) enzyme. HRP is usually used for blotting, immunoassays and immunohistochemistry methods. It is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or launch of sunshine as a byproduct of the response.

This product is on the market in a stabilized liquid sort.For substrate use of HRP-labelled protein detection we offer our Intensive ChemiLuminescence (ICLTM) gear (SKU: 20810000). ICL is a two-component substrate that accommodates a gradual luminol reply with an enhancer and a gradual peroxide buffer reply. It has a strong mild emission and a sensitivity at picogram diploma that is extraordinarily applicable for the speedy detection of HRP-conjugated proteins.

Advantages of chemiluminescent substrates over totally different detection methods accommodates plenty of exposures, blots is also reprobed, detects and quantitates a wide range of protein concentrations, and yields greatest sensitivity of any on the market approach.We moreover provide 3, 3, 5, 5-Tetramethyl benzidine (TMB, SKU: 42000012) and TMB OneTM (TMB 1) HRP substrate for HRP labelled detection. TMB is an applicable substrate for the immunoassay of horseradish peroxidase and is a noncarcinogenic by-product of benzidine with an absorbance at 450 nm. Oxidation of TMB by HRP yields a colored product. s TMB 1TM is a one factor peroxidase substrate (SKU: 21530073).

Abstract

Peanut (Arachis hypogaea) agglutinin (PNA) is extensively used as tumour arker it strongly recognises probably the most cancers specific T antigen (Galbeta1–>3GalNAc-), nonetheless not its sialylated mannequin. Nonetheless, an additional specificity within the path of Galbeta1–>4GlcNAc (LacNAc), which is not tumour specific, had been attributed to PNA.

For correct interpretation of lectin histochemical outcomes we examined PNA sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, moderately than mono- or disaccharides as ligands. Dot-blots, swap blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and totally different lectins of acknowledged specificity.

Modifications of PNA-binding glycoproteins, along with selective eradicating of O-linked oligosaccharides and remedy with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) along with uncovered T antigen (Galbeta1–>Three GalNAc-) was great as sugar moiety in glycoproteins for his or her recognition by PNA. When immobilised, melibiose was superior to lactose in PNA binding. Outcomes have been confirmed using TAG-specific human serum anti-alpha-galactoside antibody.

Biochem/Physiol Actions

PNA would not agglutinate common human erythrocytes, nonetheless strongly agglutinates neuraminidase dealt with erythrocytes. PNA has potent anti-T train very like the anti-T antibody in human sera. The lectin might be utilized to inform aside between human lymphocyte subsets.

Unit Definition

One unit will sort 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.Zero at 20 °C.

Bodily sort

Accommodates sodium citrate

Preparation Discover

Prepared from peroxidase (P8375) using a modification of a broadcast approach, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Arachis hypogaea lectin (PNA)
05-0116-10	Medicago	10 mg	EUR 116

Arachis hypogaea lectin (PNA
05-0116-1000	Medicago	1 g	Ask for price

Arachis hypogaea lectin (PNA
05-0116-50	Medicago	50 mg	EUR 314

Arachis hypogaea Arachin Ahy-3
1-CSB-EP737391ANE	Cusabio	EUR 611.00 EUR 309.00 EUR 1827.00 EUR 939.00 EUR 1218.00 EUR 397.00	100ug 10ug 1MG 200ug 500ug 50ug

Description: Recombinant Arachis hypogaea Arachin Ahy-3 expressed in E.coli

Arachis hypogaea Arachin Ahy-3
1-CSB-YP737391ANE	Cusabio	EUR 679.00 EUR 335.00 EUR 2172.00 EUR 1051.00 EUR 1442.00 EUR 435.00	100ug 10ug 1MG 200ug 500ug 50ug

Description: Recombinant Arachis hypogaea Arachin Ahy-3,partial expressed in Yeast

Arachis hypogaea Allergen Ara h 1, clone P17
1-CSB-YP331779ANE(A4)	Cusabio	EUR 586.00 EUR 299.00 EUR 2172.00 EUR 900.00 EUR 1442.00 EUR 382.00	100ug 10ug 1MG 200ug 500ug 50ug

Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in Yeast

Arachis hypogaea Allergen Ara h 1, clone P17
1-CSB-EP331779ANE(A4)	Cusabio	EUR 611.00 EUR 309.00 EUR 1827.00 EUR 939.00 EUR 1218.00 EUR 397.00	100ug 10ug 1MG 200ug 500ug 50ug

Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in E.coli

Arachis hypogaea Allergen Ara h 1, clone P17
1-CSB-EP331779ANE	Cusabio	EUR 611.00 EUR 309.00 EUR 1827.00 EUR 939.00 EUR 1218.00 EUR 397.00	100ug 10ug 1MG 200ug 500ug 50ug

Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17,partial expressed in E.coli

Arachis hypogaea Allergen Ara h 1, clone P41B
1-CSB-CF337356ANE	Cusabio	EUR 965.00 EUR 665.00 EUR 715.00	1MG 200ug 500ug

Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P41B expressed in in vitro E.coli expression system