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Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment

Heterogeneity and hierarchy inside probably the most primitive hematopoietic stem cell compartment.

Hematopoietic stem cells (HSCs) have been extensively characterised primarily based on practical definitions decided by experimental transplantation into lethally irradiated mice.

In mice, HSCs are heterogeneous with regard to self-renewal potential, in vitro colony-forming exercise, and in vivo conduct.

We tried potential isolation of HSC subsets with distinct properties amongst CD34(-/low) c-Equipment+Sca-1+Lin- (CD34-KSL) cells. CD34-KSL cells have been divided, primarily based on CD150 expression, into three fractions: CD150excessive, CD150med, and CD150neg cells.

 

In contrast with the opposite two fractions, CD150excessive cells have been considerably enriched in HSCs, with nice self-renewal potential. In vitro colony assays revealed that decreased expression of CD150 was related with diminished erythroblast/megakaryocyte differentiation potential.

 

All three fractions have been regenerated solely from CD150excessive cells in recipient mice. Utilizing single-cell transplantation research, we discovered {that a} fraction of CD150excessive cells displayed latent and barely detectable myeloid engraftment in primary-recipient mice however progressive and multilineage reconstitution in secondary-recipient mice.

 

These findings spotlight the complexity and hierarchy of reconstitution functionality, even amongst HSCs in probably the most primitive compartment.

 

 

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EZBlock? (PBS) Blocking Buffer

2128-200
EUR 137

Blocking Buffer, 10X, 250ML

X109-250ML
EUR 359

Blocking Buffer, 10X, 25ML

X109-25ML
EUR 122

TBS Blocking Buffer Pack

AR0143
EUR 65

General Blocking Buffer-BB1

KF17337
EUR 231

SynBlock Blocking Buffer-BB3

KF17339
EUR 244

Block

BSH100-02
EUR 137.21

Block

BSH100-05
EUR 146.78

Block

BSH100-1213
EUR 137.21

Block

BSH100-1232
EUR 137.21

Block

BSH100-15
EUR 137.21

Block

BSH100-150
EUR 156.35

Block

BSH100-1520
EUR 137.21

Block

BSH100-15HL
EUR 156.35

Block

BSH100-500
EUR 104.15

Block

BSH100-5MT
EUR 156.35

Block

BSH100-CV
EUR 137.21

Block

BSW02
EUR 151.93

Block

BSW05
EUR 151.93

Block

BSW10
EUR 151.93

Block

BSW13
EUR 151.93

Block

BSW15
EUR 151.93

Block

BSW1500
EUR 151.93

Block

BSW1516
EUR 183.03

Block

BSW1520
EUR 151.93

Block

BSW50
EUR 151.93

Block

BSW5MT
EUR 151.93

Block

BSWHEM
EUR 237.26

Block

BSWMT
EUR 194.99

Block

BSWNMR
EUR 481.73

Block

BSWPCR1
EUR 255.6

Block

BSWPCR2
EUR 208.55

Block

H5000-02
EUR 290.33

Block

H5000-025
EUR 591.35

Block

H5000-05
EUR 290.33

Block

H5000-12
EUR 382.55

Block

H5000-1232
EUR 382.55

Block

H5000-15
EUR 290.33

Block

H5000-150
EUR 382.55

Block

H5000-20
EUR 382.55

Block

H5000-500
EUR 382.55

Block

H5000-CMB
EUR 290.33

Block

H5000-CU
EUR 382.55

Block

H5000-WB
EUR 4288.85

Neptune 10ul barrier tip

BT10
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 100ul barrier tip

BT100
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 1000ul barrier tip

BT1000.96
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 20ul barrier tip

BT20
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 200ul barrier tip

BT200
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 300ul barrier tip

BT300
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune Assay Diluent-AD3

KF17349
EUR 244

Neptune Sample Diluent-SD3

KF17354
EUR 244

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed

ELISA-1
EUR 202

EZBlock? T20 (TBS) Blocking Buffer

2140-1000
EUR 218

EZBlock? T20 (TBS) Blocking Buffer

2140-200
EUR 120

EZBlock? T20 (PBS) Blocking Buffer

2143-1000
EUR 218

EZBlock? T20 (PBS) Blocking Buffer

2143-200
EUR 120

Blocking Buffer and Coating Stabilizer

abx090811-1000ml
EUR 523

West-Ezier Super Blocking Buffer

W3700-010
EUR 97

West-Ezier Super Blocking Buffer

W3700-050
EUR 165

West-Ezier Super Blocking Buffer

W3700-100
EUR 246

PBS Blocking buffer with BSA

SB0626
EUR 83.93

TBS blocking buffer with BSA

SD8113
EUR 83.93

Permeabilization and Blocking Buffer (5X)

22017
EUR 149
Description: Minimum order quantity: 1 unit of 100mL

TrueBlack WB Blocking Buffer Kit

23013
EUR 347
Description: Minimum order quantity: 1 unit of 1kit

TrueBlack WB Blocking Buffer Kit

23013-T
EUR 125
Description: Minimum order quantity: 1 unit of 1kit

3 Blocking Buffer Sample Pack

KF17340
EUR 205

Peroxide Block

ACA015
EUR 67

Peroxide Block

ACA125
EUR 76

Peroxide Block

ACA500
EUR 101

Peroxide Block

ACA999
EUR 125

Super Block

AAA125
EUR 74

Super Block

AAA500
EUR 101

Super Block

AAA999
EUR 124

EZ Block

EZB125
EUR 93

EZ Block

EZB500
EUR 127

EZ Block

EZB999
EUR 173

Pro-Block

PBK-20000
EUR 1210

Pro-Block

PBK010
EUR 676

Pro-Block

PBK125
EUR 74

Pro-Block

PBK500
EUR 101

Pro-Block

PBK999
EUR 124

Block-Free ELISA Reagent

M4102-250
EUR 452

Block-Free ELISA Reagent

M4102-50
EUR 147

Neptune 1000-1250ul barrier tip

BT1250
EUR 59
Description: Extended Length, Low retention, Racked, Pre-Sterile, Sample Saving Surface

ChonBlock Blocking/Sample Dilution ELISA Buffer, 100 ml

9068
EUR 129.75
Description: ChonBlock Blocking/Sample Dilution ELISA Buffer

ELISA Plate Blocking Buffer concentrate (10X) BSA-based

80060
EUR 202

ELISA Plate Blocking Buffer concentrate (10X) milk-based

80062
EUR 202

EZBlock? Blocking Buffer and Signal Enhancer

M4104-100
EUR 153

EZBlock? Blocking Buffer and Signal Enhancer

M4104-500
EUR 359

Casein-based PBS Blocking Buffer Pack

AR0182
EUR 70

Casein-based TBS Blocking Buffer Pack

AR0183
EUR 70

Blocking Buffer for ICC and IHC

SF40011
EUR 128

Neptune 10ul extended length barrier tip

BT10XLS3
EUR 59
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Block, PCR plate, For 1-block dry bath

BCM1412-BSWPCR1
EUR 363.2

Blocking Buffer for Lateral Flow (PBS based)

abx090808-500ml
EUR 384

PBS Blocking buffer with non fat milk

SB0625
EUR 67.4

 

Spermatogonial stem cells share some, however not all, phenotypic and practical traits with different stem cells.

 

Spermatogonial stem cells (SSCs) are chargeable for sustaining spermatogenesis all through life within the male by steady manufacturing of daughter cells that differentiate into spermatozoa.

 

Nevertheless, no distinctive phenotypic markers to determine SSCs have been described. On this examine, the SSC floor phenotype was characterised by utilizing movement cytometric cell sorting in conjunction with a transplantation practical assay for SSCs.

 

Extremely enriched stem cell exercise was discovered within the MHC class I (MHC-I)-Thy-1+c-package– cell fraction of the mouse cryptorchid testis. There was little or no stem cell exercise in some other fraction.

 

The antigenic phenotype of the MHC-I-Thy-1+c-package– SSCs was alpha6-integrin+CD24+alphavintegrin-Sca-1-CD34-. Subsequently, testis facet inhabitants (SP) cells, that are outlined by a Hoechst dye efflux assay, have been recognized.

 

Their floor phenotype was discovered to be MHC-I+Thy-1-Sca-1+, and the transplantation assay demonstrated that the testis SP and SSCs are distinct populations. In a number of different tissues, the SP has been proven to include stem cells, however we discovered that this attribute doesn’t outline SSCs.

 

The identification of a floor phenotype that enables manufacturing of a extremely enriched SSC inhabitants will facilitate practical and genomic research and allow additional comparability with different stem cells.

 

Isolation of multipotent progenitor cells from human fetal liver able to differentiating into liver and mesenchymal lineages.

 

Little is thought concerning the differentiation capabilities of nonhematopoietic cells of the human fetal liver. We report the isolation and characterization of a human fetal liver multipotent progenitor cell (hFLMPC) inhabitants succesful of differentiating into liver and mesenchymal cell lineages.

 

Human fetal livers (74-108 days of gestation) have been dissociated and maintained in tradition. We handled the colonies with geneticin and mechanically remoted hFLMPCs, which have been stored in an undifferentiated state by culturing on feeder layers.

 

We derived daughter colonies by serial dilution, verifying monoclonality utilizing the Humara assay. hFLMPCs, which have been maintained in tradition for as much as 100 inhabitants doublings, have a excessive self-renewal functionality with a doubling time of 46 h. The immunophenotype is: CD34+, CD90+, c-package+, EPCAM+, c-met+, SSEA-4+, CK18+, CK19+, albumin-, alpha-fetoprotein-, CD44h+, and vimentin+. Passage 1 (P1) and P10 cells have an identical morphology, immunophenotype, telomere size, and differentiation capability.

 

Positioned in acceptable media, hFLMPCs differentiate into hepatocytes and bile duct cells, in addition to into fats, bone, cartilage, and endothelial cells. Our outcomes recommend that hFLMPCs are mesenchymal-epithelial transitional cells, most likely derived from mesendoderm.

 

hFLMPCs survive and differentiate into practical hepatocytes in vivo when transplanted into animal fashions of liver illness. hFLMPCs are a priceless instrument for the examine of human liver improvement, liver harm, and hepatic repopulation.

 

LDH Cytotoxicity Assay Kit

K6330400
EUR 306
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Bioluminescence Cytotoxicity Assay Kit

K312-500
EUR 245

LDH-Cytotoxicity Colorimetric Assay Kit

K311-400
EUR 387

CytoSelect LDH Cytotoxicity Assay Kit

CBA-241
EUR 403
Description: Cell Biolabs? CytoSelect LDH Cytotoxicity Assay Kit provides a colorimetric format for measuring and monitoring cell cytotoxicity.  The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates.  Cells can be plated and then treated with compounds or agents that affect cell viability.  Upon cell death, lactate dehydrogenase (LDH), a soluble enzyme found in the cytoplasm, is released into the growth media.  The growth media is then transferred to another plate and the released LDH is then detected with cytotoxicity reagent.  In the presence of lactate substrate (included in the LDH Cytotoxicity Reagent) LDH converts lactate to pyruvate and generates nicotinamide adenine dinucleotide (NADH).   The WST-1 molecule, also present in the LDH Cytotoxicity Reagent, is converted from WST-1 to the orange formazan form.  An increase in cell cytotoxicity is accompanied by increased LDH release and increased colorimetric signal.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues, depending on LDH expression levels.  The LDH Cytotoxicity Reagent can be used to detect cytotoxicity in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

LDH-Cytotoxicity Colorimetric Assay Kit

K2025-400
EUR 390

AK Bioluminescence Cytotoxicity Assay Kit

K2026-500
EUR 266

Acid Phosphatase Cell Cytotoxicity Assay Kit

K937-500
EUR 370

PicoProbe? LDH-Cytotoxicity Fluorometric Assay Kit

K314-500
EUR 419

Neutral Red Cell Cytotoxicity Assay Kit

K447-1000
EUR 316

LDH-Cytotoxicity Colorimetric Assay Kit II

K313-500
EUR 419

Crystal Violet Cell Cytotoxicity Assay Kit

K329-1000
EUR 316

LDH-Cytotoxicity Colorimetric Assay Kit II

K2027-500
EUR 418

Basic Xylanase Assay Kit

abx298857-100Assays
EUR 457

QuantiChrom? LDH Cytotoxicity Assay Kit (C2LD-100)

C2LD-100
EUR 127.43
Description: Quantitative determination of cytotoxicity based on cellular lactate dehydrogenase release into cell culture medium by colorimetric (565nm) method. Procedure: 30 min. Kit size: 100 tests. Detection limit: 2 U/L. Shelf life: 6 months. Shipping: ambient tem

WST-NR-CV Combined Cytotoxicity Assay Kit

K543-1000
EUR 675

Sulforhodamine B Cell Cytotoxicity Assay Kit (Colorimetric)

K943-1000
EUR 316

MTT Cell Proliferation and Cytotoxicity Assay Kit

AR1156
EUR 96

MTT Cell Proliferation and Cytotoxicity Assay Kit

abx090676-500tests
EUR 237

Cell Proliferation and Cytotoxicity MTT Assay Kit

C0210-100
EUR 165

Cell Proliferation and Cytotoxicity MTT Assay Kit

C0210-500
EUR 300

Cell Proliferation and Cytotoxicity MTT Assay Kit

C0210-501
EUR 1238

CytoX-Red Cell Proliferation/Cytotoxicity Assay Kit

OP-0004
EUR 395.1
Description: reagents widely cited

CytoX-Violet Cell Proliferation/Cytotoxicity Assay Kit

OP-0005
EUR 395.1
Description: fast delivery possible

NATtrol Zika Virus Stock (Qualitative) (1 mL)

TEST
EUR 1106.64
Description: Please contact Gentaur in order to receive the datasheet of the product.

Peroxide assay, colorimetric micro assay kit, 96 test, Quantitative

1350-POX-1
EUR 408

Peroxidase assay, colorimetric micro assay kit, 96 test, Quantitative

1360-PAS-1
EUR 529

CytoSelect Cell Viability and Cytotoxicity Assay

CBA-240
EUR 392
Description: The CytoSelect Cell Viability and Cytotoxicity Assay Kit provides a simple format for monitoring cell viability via metabolic activity. Live cells are detected with either MTT (colorimetric detection) or Calcein AM (fluorometric detection). Dead cells are detected by EthD-1 reagent (fluorometric). All 3 detection reagents are included, along with Saponin (a cell death initiator). Prior to the assay, cells may be treated with compounds or agents that affect cell viability. This kit is suitable for eukaryotic cells, not yeast or bacteria.

Cell Meter™ Colorimetric Cell Cytotoxicity Assay Kit

22780
EUR 219

Cell Meter™ Fluorimetric Cell Cytotoxicity Assay Kit

22781
EUR 219

Neutral Red Cell Proliferation and Cytotoxicity Assay Kit

AR1157
EUR 101

WST-1 Cell Proliferation and Cytotoxicity Assay Kit

AR1158
EUR 86

WST-1 Cell Proliferation and Cytotoxicity Assay Kit

AR1159
EUR 131

Glutathione (GSH) Assay, Enzymatic colorimetric assay kit, 96 test, Quantitative

1250-GSH-1
EUR 408

Glutathione Peroxidase Assay (GPX), Enzymatic colorimetric assay kit, 96 test, Quantitative

1240-GPX-1
EUR 590

Cell-Mediated Cytotoxicity Fluorometric Assay Kit (7-AAD/CFSE)

K315-100
EUR 327

Cell Cytotoxicity Assay Kit II (WST-SRB-Crystal Violet)

K173-1000
EUR 707

Glucose colorimetric micro assay kit, 96 test, Quantitative

1380-GLC-1
EUR 286

Cholesterol colorimetric micro assay kit, 96 test, Quantitative

1390-CHL-1
EUR 529

Cholesterol Fluorometric micro assay kit, 96 test, Quantitative

1395-CHL-1
EUR 347

Glycogen fluorometric micro assay kit, 96 test, Quantitative

1530-GLY-1
EUR 529

Hemoglobin colorimetric micro assay kit, 96 test, Quantitative

1540-HEM-1
EUR 286

Triglyceride colorimetric micro assay kit, 96 test, Quantitative

1550-TGL-1
EUR 286

BCA Protein Assay Kit

SK3021
EUR 106.55

Bradford Protein Assay Kit

SK3031
EUR 74.36

Lowry Protein Assay Kit

SK4031
EUR 72.62

FGF-basic, Fibroblast Growth Factor-basic, human

RC215-13
EUR 152.66

FGF-basic, Fibroblast Growth Factor-basic, rat

RC255-13
EUR 152.66

FGF-basic, Fibroblast Growth Factor-basic, bovine

RC265-13
EUR 152.66

Viability/Cytotoxicity Assay kit for Bacteria Live and Dead Cells

30027
EUR 356
Description: Minimum order quantity: 1 unit of 1KIT

Phosphoprotein Phosphate Estimation Assay Kit

BSP061
EUR 91.76

Micro BCA Protein Assay Kit

SK3061
EUR 92.63

FGF-basic, Fibroblast Growth Factor-basic, murine (mouse)

RC235-13
EUR 152.66

Basic fuchsin, certified

BB1111
EUR 56.09

Basic fuchsin, hydrochloride

BD0070
EUR 63.05

BCA Protein Assay Kit (Test Tube) - Reducing Agent Compatible

K819-250
EUR 327

Nitric Oxide Synthase (NOS) enzyme assay, colorimetric micro assay kit, 96 test, Quantitative

1290-NOS-1
EUR 651

Peroxide Quantitative Assay Kit (Lipid-Compatible)

BSP067
EUR 91.76

Peroxide Quantitative Assay Kit (Water compatible)

BSP069
EUR 91.76

Lead (II) carbonate basic

LB0559
EUR 59.57

Rosaniline chloride (Fuchsin basic)

R2770
EUR 60.44

Ketone Body Enzymatic colorimetric micro assay kit, 96 test, Quantitative

1260-KTN-1
EUR 408

Better Bradford Protein Assay Kit (Improved linearity)

SK3041
EUR 83.06

Modified Lowry Protein Assay Kit (Improved accuracy)

SK4041
EUR 74.36

Stable Lowry Protein Assay Kit (Improved stability)

SK4051
EUR 83.06

Total Antioxidant Capacity (TAC), colorimetric micro assay kit, 96 test, Quantitative

1370-TAS-1
EUR 529

Block, 20x10mm test tubes or 20x2.0ml centrifuge tubes

BCM1412-BSW10
EUR 194.34

Block, 20 x 12mm or 13mm test tubes

BCM1412-BSW13
EUR 194.34

Block, 12 x 15mm or 16mm test tubes

BCM1412-BSW1516
EUR 194.34

Better BCA Protein Assay Kit (Reducing agent compatible)

SK3051
EUR 114.38

Human NKp46/NCR1(Natural cytotoxicity triggering receptor 1) ELISA Kit

EH4292
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 3.75pg/ml

Human NKp44/NCR2(Natural cytotoxicity triggering receptor 2) ELISA Kit

EH4293
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

Human NKp30/NCR3(Natural cytotoxicity triggering receptor 3) ELISA Kit

EH4294
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

Anti-SARS-CoV-2 Neutralization Antibody Test Kit (Enzyme-linked immunosorbent assay)

CP04012 Ask for price
Description: It is a semi quantitative competition ELISA kit to detect the Anti-SARS-CoV-2 Neutralization Antibody in human serum or plasma. The micro test plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 neutralization antibody in the standard & sample diluent pretreated samples or controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD). After 37℃incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.

Ascorbic Acid/Ascorbate (Vitmain C) fluorometric micro assay kit, 96 test, Quantitative

1520-ASC-1
EUR 529

Thiobarbituric acid reactive substances (TBARS) colorimetric micro assay kit, 96 test, Quantitative

1270-TBA-1
EUR 408

Nitric Oxide (NO/nitrate/nitrite) colorimetric micro assay kit, 96 test, Quantitative

1280-NOX-1
EUR 529

Cytotoxicity Control (saponin)

Z5030008
EUR 249
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Mouse MBP(Myelin Basic Protein) ELISA Kit

EM1202
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml

Porcine MBP(Myelin Basic Protein) ELISA Kit

EP0109
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Pig;Sensitivity: 0.188 ng/ml

Human MBP(myelin basic protein) ELISA Kit

EH4023
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human CXCL7(Platelet basic protein) ELISA Kit

EH0007
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

Rat Mbp(Myelin basic protein) ELISA Kit

ER0315
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.094 ng/ml

Melamine Rapid Test Kit

abx092011-50tests
EUR 370

NDV rapid test kit

RG15-03
EUR 139.05
Description: Please check the datasheet of NDV rapid test kit before using the test.

Frit Kit

FRIT-KIT
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit

PACK-KIT
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

anti- Myelin basic protein antibody

FNab05465
EUR 505.25
Description: Antibody raised against Myelin basic protein

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