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Heterogeneity and hierarchy within the most primitive hematopoietic stem cell compartment

Heterogeneity and hierarchy inside probably the most primitive hematopoietic stem cell compartment.

Hematopoietic stem cells (HSCs) have been extensively characterised primarily based on practical definitions decided by experimental transplantation into lethally irradiated mice.

In mice, HSCs are heterogeneous with regard to self-renewal potential, in vitro colony-forming exercise, and in vivo conduct.

We tried potential isolation of HSC subsets with distinct properties amongst CD34(-/low) c-Equipment+Sca-1+Lin- (CD34-KSL) cells. CD34-KSL cells have been divided, primarily based on CD150 expression, into three fractions: CD150excessive, CD150med, and CD150neg cells.

 

In contrast with the opposite two fractions, CD150excessive cells have been considerably enriched in HSCs, with nice self-renewal potential. In vitro colony assays revealed that decreased expression of CD150 was related with diminished erythroblast/megakaryocyte differentiation potential.

 

All three fractions have been regenerated solely from CD150excessive cells in recipient mice. Utilizing single-cell transplantation research, we discovered {that a} fraction of CD150excessive cells displayed latent and barely detectable myeloid engraftment in primary-recipient mice however progressive and multilineage reconstitution in secondary-recipient mice.

 

These findings spotlight the complexity and hierarchy of reconstitution functionality, even amongst HSCs in probably the most primitive compartment.

 

 

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EZBlock? (PBS) Blocking Buffer

2128-200
EUR 164.4

TBS Blocking Buffer Pack

AR0143
EUR 78

General Blocking Buffer-BB1

KF17337
EUR 277.2

SynBlock Blocking Buffer-BB3

KF17339
EUR 292.8

Blocking Buffer, 10X, 250ML

X109-250ML
EUR 430.8

Blocking Buffer, 10X, 25ML

X109-25ML
EUR 146.4

Neptune 10ul barrier tip

BT10
EUR 70.8
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 100ul barrier tip

BT100
EUR 70.8
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 1000ul barrier tip

BT1000.96
EUR 70.8
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 20ul barrier tip

BT20
EUR 70.8
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 200ul barrier tip

BT200
EUR 70.8
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 300ul barrier tip

BT300
EUR 70.8
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune Assay Diluent-AD3

KF17349
EUR 292.8

Neptune Sample Diluent-SD3

KF17354
EUR 292.8

Block

BSH100-02
EUR 164.65

Block

BSH100-05
EUR 176.14

Block

BSH100-1213
EUR 164.65

Block

BSH100-1232
EUR 164.65

Block

BSH100-15
EUR 164.65

Block

BSH100-150
EUR 187.62

Block

BSH100-1520
EUR 164.65

Block

BSH100-15HL
EUR 187.62

Block

BSH100-500
EUR 124.98

Block

BSH100-5MT
EUR 187.62

Block

BSH100-CV
EUR 164.65

Block

BSW02
EUR 182.32

Block

BSW05
EUR 182.32

Block

BSW10
EUR 182.32

Block

BSW13
EUR 182.32

Block

BSW15
EUR 182.32

Block

BSW1500
EUR 182.32

Block

BSW1516
EUR 219.64

Block

BSW1520
EUR 182.32

Block

BSW50
EUR 182.32

Block

BSW5MT
EUR 182.32

Block

BSWHEM
EUR 284.71

Block

BSWMT
EUR 233.99

Block

BSWNMR
EUR 578.08

Block

BSWPCR1
EUR 306.72

Block

BSWPCR2
EUR 250.26

Block

H5000-02
EUR 348.4

Block

H5000-025
EUR 709.62

Block

H5000-05
EUR 348.4

Block

H5000-12
EUR 459.06

Block

H5000-1232
EUR 459.06

Block

H5000-15
EUR 348.4

Block

H5000-150
EUR 459.06

Block

H5000-20
EUR 459.06

Block

H5000-500
EUR 459.06

Block

H5000-CMB
EUR 348.4

Block

H5000-CU
EUR 459.06

Block

H5000-WB
EUR 5146.62

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed

ELISA-1
EUR 242.4

EZBlock? T20 (TBS) Blocking Buffer

2140-1000
EUR 261.6

EZBlock? T20 (TBS) Blocking Buffer

2140-200
EUR 144

EZBlock? T20 (PBS) Blocking Buffer

2143-1000
EUR 261.6

EZBlock? T20 (PBS) Blocking Buffer

2143-200
EUR 144

TrueBlack WB Blocking Buffer Kit

23013
EUR 416.4
Description: Minimum order quantity: 1 unit of 1kit

TrueBlack WB Blocking Buffer Kit

23013-T
EUR 150
Description: Minimum order quantity: 1 unit of 1kit

Blocking Buffer and Coating Stabilizer

abx090811-1000ml
EUR 627.6

3 Blocking Buffer Sample Pack

KF17340
EUR 246

10 x Universal Blocking Buffer

GR103005
EUR 154.8

TBS blocking buffer with BSA

SD8113
EUR 100.72

PBS Blocking buffer with BSA

SB0626
EUR 100.72

West-Ezier Super Blocking Buffer

W3700-010
EUR 116.4

West-Ezier Super Blocking Buffer

W3700-050
EUR 198

West-Ezier Super Blocking Buffer

W3700-100
EUR 295.2

Neptune 1000-1250ul barrier tip

BT1250
EUR 70.8
Description: Extended Length, Low retention, Racked, Pre-Sterile, Sample Saving Surface

Block-Free ELISA Reagent

M4102-250
EUR 542.4

Block-Free ELISA Reagent

M4102-50
EUR 176.4

Super Block

AAA125
EUR 88.8

Super Block

AAA500
EUR 121.2

Super Block

AAA999
EUR 148.8

Peroxide Block

ACA015
EUR 80.4

Peroxide Block

ACA125
EUR 91.2

Peroxide Block

ACA500
EUR 121.2

Peroxide Block

ACA999
EUR 150

EZ Block

EZB125
EUR 111.6

EZ Block

EZB500
EUR 152.4

EZ Block

EZB999
EUR 207.6

Pro-Block

PBK-20000
EUR 1452

Pro-Block

PBK010
EUR 811.2

Pro-Block

PBK125
EUR 88.8

Pro-Block

PBK500
EUR 121.2

Pro-Block

PBK999
EUR 148.8

ChonBlock Blocking/Sample Dilution ELISA Buffer, 100 ml

9068
EUR 155.7
Description: ChonBlock Blocking/Sample Dilution ELISA Buffer

ELISA Plate Blocking Buffer concentrate (10X) BSA-based

80060
EUR 242.4

Casein-based PBS Blocking Buffer Pack

AR0182
EUR 84

Casein-based TBS Blocking Buffer Pack

AR0183
EUR 84

EZBlock? Blocking Buffer and Signal Enhancer

M4104-100
EUR 183.6

EZBlock? Blocking Buffer and Signal Enhancer

M4104-500
EUR 430.8

Blocking Buffer for ICC and IHC

SF40011
EUR 153.6

Neptune 10ul extended length barrier tip

BT10XLS3
EUR 70.8
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Block, PCR plate, For 1-block dry bath

BCM1412-BSWPCR1
EUR 435.84

Biotin Block Kit

B3810-002
EUR 262.8

Biotin Block Kit

B3810-005
EUR 489.6

HiQTM Immuno Block

I3211-010
EUR 160.8

 

Spermatogonial stem cells share some, however not all, phenotypic and practical traits with different stem cells.

 

Spermatogonial stem cells (SSCs) are chargeable for sustaining spermatogenesis all through life within the male by steady manufacturing of daughter cells that differentiate into spermatozoa.

 

Nevertheless, no distinctive phenotypic markers to determine SSCs have been described. On this examine, the SSC floor phenotype was characterised by utilizing movement cytometric cell sorting in conjunction with a transplantation practical assay for SSCs.

 

Extremely enriched stem cell exercise was discovered within the MHC class I (MHC-I)-Thy-1+c-package– cell fraction of the mouse cryptorchid testis. There was little or no stem cell exercise in some other fraction.

 

The antigenic phenotype of the MHC-I-Thy-1+c-package– SSCs was alpha6-integrin+CD24+alphavintegrin-Sca-1-CD34-. Subsequently, testis facet inhabitants (SP) cells, that are outlined by a Hoechst dye efflux assay, have been recognized.

 

Their floor phenotype was discovered to be MHC-I+Thy-1-Sca-1+, and the transplantation assay demonstrated that the testis SP and SSCs are distinct populations. In a number of different tissues, the SP has been proven to include stem cells, however we discovered that this attribute doesn’t outline SSCs.

 

The identification of a floor phenotype that enables manufacturing of a extremely enriched SSC inhabitants will facilitate practical and genomic research and allow additional comparability with different stem cells.

 

Isolation of multipotent progenitor cells from human fetal liver able to differentiating into liver and mesenchymal lineages.

 

Little is thought concerning the differentiation capabilities of nonhematopoietic cells of the human fetal liver. We report the isolation and characterization of a human fetal liver multipotent progenitor cell (hFLMPC) inhabitants succesful of differentiating into liver and mesenchymal cell lineages.

 

Human fetal livers (74-108 days of gestation) have been dissociated and maintained in tradition. We handled the colonies with geneticin and mechanically remoted hFLMPCs, which have been stored in an undifferentiated state by culturing on feeder layers.

 

We derived daughter colonies by serial dilution, verifying monoclonality utilizing the Humara assay. hFLMPCs, which have been maintained in tradition for as much as 100 inhabitants doublings, have a excessive self-renewal functionality with a doubling time of 46 h. The immunophenotype is: CD34+, CD90+, c-package+, EPCAM+, c-met+, SSEA-4+, CK18+, CK19+, albumin-, alpha-fetoprotein-, CD44h+, and vimentin+. Passage 1 (P1) and P10 cells have an identical morphology, immunophenotype, telomere size, and differentiation capability.

 

Positioned in acceptable media, hFLMPCs differentiate into hepatocytes and bile duct cells, in addition to into fats, bone, cartilage, and endothelial cells. Our outcomes recommend that hFLMPCs are mesenchymal-epithelial transitional cells, most likely derived from mesendoderm.

 

hFLMPCs survive and differentiate into practical hepatocytes in vivo when transplanted into animal fashions of liver illness. hFLMPCs are a priceless instrument for the examine of human liver improvement, liver harm, and hepatic repopulation.

 

Total Cytotoxicity Test Kits

CT17003
EUR 615.6

Total Cytotoxicity Test Kits

CT17004
EUR 975.6

CytoSelect LDH Cytotoxicity Assay Kit

CBA-241
EUR 483.6
Description: Cell Biolabs? CytoSelect LDH Cytotoxicity Assay Kit provides a colorimetric format for measuring and monitoring cell cytotoxicity.  The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates.  Cells can be plated and then treated with compounds or agents that affect cell viability.  Upon cell death, lactate dehydrogenase (LDH), a soluble enzyme found in the cytoplasm, is released into the growth media.  The growth media is then transferred to another plate and the released LDH is then detected with cytotoxicity reagent.  In the presence of lactate substrate (included in the LDH Cytotoxicity Reagent) LDH converts lactate to pyruvate and generates nicotinamide adenine dinucleotide (NADH).   The WST-1 molecule, also present in the LDH Cytotoxicity Reagent, is converted from WST-1 to the orange formazan form.  An increase in cell cytotoxicity is accompanied by increased LDH release and increased colorimetric signal.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues, depending on LDH expression levels.  The LDH Cytotoxicity Reagent can be used to detect cytotoxicity in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

LDH-Cytotoxicity Colorimetric Assay Kit

K311-400
EUR 464.4

LDH-Cytotoxicity Colorimetric Assay Kit

K2025-400
EUR 468

AK Bioluminescence Cytotoxicity Assay Kit

K2026-500
EUR 319.2

Acid Phosphatase Cell Cytotoxicity Assay Kit

K937-500
EUR 444

Crystal Violet Cell Cytotoxicity Assay Kit

K329-1000
EUR 379.2

LDH-Cytotoxicity Colorimetric Assay Kit II

K313-500
EUR 502.8

PicoProbe? LDH-Cytotoxicity Fluorometric Assay Kit

K314-500
EUR 502.8

Genorise® LDH Cytotoxicity Assay Kit

GR107034
EUR 346.8

Neutral Red Cell Cytotoxicity Assay Kit

K447-1000
EUR 379.2

LDH-Cytotoxicity Colorimetric Assay Kit II

K2027-500
EUR 501.6

Basic Xylanase Assay Kit

abx298857-100Assays
EUR 548.4

Cell Proliferation and Cytotoxicity MTT Assay Kit

C0210-100
EUR 198

Cell Proliferation and Cytotoxicity MTT Assay Kit

C0210-500
EUR 360

Cell Proliferation and Cytotoxicity MTT Assay Kit

C0210-501
EUR 1485.6

QuantiChrom? LDH Cytotoxicity Assay Kit (C2LD-100)

C2LD-100
EUR 152.92
Description: Quantitative determination of cytotoxicity based on cellular lactate dehydrogenase release into cell culture medium by colorimetric (565nm) method. Procedure: 30 min. Kit size: 100 tests. Detection limit: 2 U/L. Shelf life: 6 months. Shipping: ambient tem

MTT Cell Proliferation and Cytotoxicity Assay Kit

abx090676-500tests
EUR 284.4

MTT Cell Proliferation and Cytotoxicity Assay Kit

AR1156
EUR 115.2

Sulforhodamine B Cell Cytotoxicity Assay Kit (Colorimetric)

K943-1000
EUR 379.2

CytoX-Red Cell Proliferation/Cytotoxicity Assay Kit

OP-0004
EUR 474.12
Description: reagents widely cited

CytoX-Violet Cell Proliferation/Cytotoxicity Assay Kit

OP-0005
EUR 474.12
Description: fast delivery possible

WST-NR-CV Combined Cytotoxicity Assay Kit

K543-1000
EUR 810

Peroxide assay, colorimetric micro assay kit, 96 test, Quantitative

1350-POX-1
EUR 489.6

Peroxidase assay, colorimetric micro assay kit, 96 test, Quantitative

1360-PAS-1
EUR 634.8

CytoSelect Cell Viability and Cytotoxicity Assay

CBA-240
EUR 470.4
Description: The CytoSelect Cell Viability and Cytotoxicity Assay Kit provides a simple format for monitoring cell viability via metabolic activity. Live cells are detected with either MTT (colorimetric detection) or Calcein AM (fluorometric detection). Dead cells are detected by EthD-1 reagent (fluorometric). All 3 detection reagents are included, along with Saponin (a cell death initiator). Prior to the assay, cells may be treated with compounds or agents that affect cell viability. This kit is suitable for eukaryotic cells, not yeast or bacteria.

Glutathione (GSH) Assay, Enzymatic colorimetric assay kit, 96 test, Quantitative

1250-GSH-1
EUR 489.6

Cell Meter™ Colorimetric Cell Cytotoxicity Assay Kit

22780
EUR 262.8

Cell Meter™ Fluorimetric Cell Cytotoxicity Assay Kit

22781
EUR 262.8

Neutral Red Cell Proliferation and Cytotoxicity Assay Kit

AR1157
EUR 121.2

WST-1 Cell Proliferation and Cytotoxicity Assay Kit

AR1158
EUR 103.2

WST-1 Cell Proliferation and Cytotoxicity Assay Kit

AR1159
EUR 157.2

NATtrol Zika Virus Stock (Qualitative) (1 mL)

TEST
EUR 1327.97
Description: Please contact Gentaur in order to receive the datasheet of the product.

Glutathione Peroxidase Assay (GPX), Enzymatic colorimetric assay kit, 96 test, Quantitative

1240-GPX-1
EUR 708

BCA Protein Assay Kit

SK3021
EUR 127.86

Bradford Protein Assay Kit

SK3031
EUR 89.23

Lowry Protein Assay Kit

SK4031
EUR 87.14

Cell-Mediated Cytotoxicity Fluorometric Assay Kit (7-AAD/CFSE)

K315-100
EUR 392.4

Cell Cytotoxicity Assay Kit II (WST-SRB-Crystal Violet)

K173-1000
EUR 848.4

Glucose colorimetric micro assay kit, 96 test, Quantitative

1380-GLC-1
EUR 343.2

Cholesterol colorimetric micro assay kit, 96 test, Quantitative

1390-CHL-1
EUR 634.8

Cholesterol Fluorometric micro assay kit, 96 test, Quantitative

1395-CHL-1
EUR 416.4

Glycogen fluorometric micro assay kit, 96 test, Quantitative

1530-GLY-1
EUR 634.8

Hemoglobin colorimetric micro assay kit, 96 test, Quantitative

1540-HEM-1
EUR 343.2

Triglyceride colorimetric micro assay kit, 96 test, Quantitative

1550-TGL-1
EUR 343.2

FGF-basic, Fibroblast Growth Factor-basic, human

RC215-13
EUR 183.19

FGF-basic, Fibroblast Growth Factor-basic, rat

RC255-13
EUR 183.19

FGF-basic, Fibroblast Growth Factor-basic, bovine

RC265-13
EUR 183.19

Phosphoprotein Phosphate Estimation Assay Kit

BSP061
EUR 110.11

Micro BCA Protein Assay Kit

SK3061
EUR 111.16

Viability/Cytotoxicity Assay kit for Bacteria Live and Dead Cells

30027
EUR 427.2
Description: Minimum order quantity: 1 unit of 1KIT

FGF-basic, Fibroblast Growth Factor-basic, murine (mouse)

RC235-13
EUR 183.19

Basic fuchsin, certified

BB1111
EUR 67.31

Basic fuchsin, hydrochloride

BD0070
EUR 75.66

BCA Protein Assay Kit (Test Tube) - Reducing Agent Compatible

K819-250
EUR 392.4

Nitric Oxide Synthase (NOS) enzyme assay, colorimetric micro assay kit, 96 test, Quantitative

1290-NOS-1
EUR 781.2

Peroxide Quantitative Assay Kit (Lipid-Compatible)

BSP067
EUR 110.11

Peroxide Quantitative Assay Kit (Water compatible)

BSP069
EUR 110.11

Lead (II) carbonate basic

LB0559
EUR 71.48

Rosaniline chloride (Fuchsin basic)

R2770
EUR 72.53

Ketone Body Enzymatic colorimetric micro assay kit, 96 test, Quantitative

1260-KTN-1
EUR 489.6

Better Bradford Protein Assay Kit (Improved linearity)

SK3041
EUR 99.67

Modified Lowry Protein Assay Kit (Improved accuracy)

SK4041
EUR 89.23

Stable Lowry Protein Assay Kit (Improved stability)

SK4051
EUR 99.67

Better BCA Protein Assay Kit (Reducing agent compatible)

SK3051
EUR 137.26

Block, 20x10mm test tubes or 20x2.0ml centrifuge tubes

BCM1412-BSW10
EUR 233.21

Block, 20 x 12mm or 13mm test tubes

BCM1412-BSW13
EUR 233.21

Block, 12 x 15mm or 16mm test tubes

BCM1412-BSW1516
EUR 233.21

Human NKp46/NCR1(Natural cytotoxicity triggering receptor 1) ELISA Kit

EH4292
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 3.75pg/ml

Human NKp44/NCR2(Natural cytotoxicity triggering receptor 2) ELISA Kit

EH4293
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

Human NKp30/NCR3(Natural cytotoxicity triggering receptor 3) ELISA Kit

EH4294
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

Total Antioxidant Capacity (TAC), colorimetric micro assay kit, 96 test, Quantitative

1370-TAS-1
EUR 634.8

Mouse MBP(Myelin Basic Protein) ELISA Kit

EM1202
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml

Human MBP(myelin basic protein) ELISA Kit

EH4023
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Rat Mbp(Myelin basic protein) ELISA Kit

ER0315
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.094 ng/ml

Porcine MBP(Myelin Basic Protein) ELISA Kit

EP0109
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Pig;Sensitivity: 0.188 ng/ml

Human CXCL7(Platelet basic protein) ELISA Kit

EH0007
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 9.375pg/ml

Anti-SARS-CoV-2 Neutralization Antibody Test Kit (Enzyme-linked immunosorbent assay)

CP04012 Ask for price
Description: It is a semi quantitative competition ELISA kit to detect the Anti-SARS-CoV-2 Neutralization Antibody in human serum or plasma. The micro test plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 neutralization antibody in the standard & sample diluent pretreated samples or controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD). After 37℃incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.

Ascorbic Acid/Ascorbate (Vitmain C) fluorometric micro assay kit, 96 test, Quantitative

1520-ASC-1
EUR 634.8

Thiobarbituric acid reactive substances (TBARS) colorimetric micro assay kit, 96 test, Quantitative

1270-TBA-1
EUR 489.6

Nitric Oxide (NO/nitrate/nitrite) colorimetric micro assay kit, 96 test, Quantitative

1280-NOX-1
EUR 634.8

Cytotoxicity Control (saponin)

Z5030008
EUR 298.8
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Frit Kit

FRIT-KIT
EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Melamine Rapid Test Kit

abx092011-50tests
EUR 444

NDV rapid test kit

RG15-03
EUR 166.86
Description: Please check the datasheet of NDV rapid test kit before using the test.

anti- Myelin basic protein antibody

FNab05465
EUR 606.3
Description: Antibody raised against Myelin basic protein

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