DL-Serine Assay Kit (Fluorometric)

DESCRIPTION

Serine is without doubt one of the 20 naturally-occurring amino acids utilized by all organisms within the biosynthesis of proteins. Having a single chiral heart, serine can exist as both of two stereoisomers (L-Serine and D-Serine). Along with its position as a protein constructing block, L-Serine serves as a precursor to many very important biomolecules, together with phosphatidylserine, sphingomyelin and the amino acids glycine and cysteine.

DL Serine Standard Curve
DL Serine Customary Curve

The non-proteogenic isomer D-Serine can also be synthesized from L-Serine within the mammalian mind. Whereas most D-amino acids are solely current in hint quantities in mammals, D-Serine is an important neurotransmitter that acts as a co-agonist of synaptic N-methyl-D-aspartate (NMDA) sort glutamate-gated ion channels. Impaired D-Serine metabolism might contribute to a number of neuropsychiatric issues, together with despair, schizophrenia, epilepsy and dementia. BioVision’s DL-Serine Assay Equipment permits for quantification of each L- and D-Serine in organic fluids and tissues. The assay is predicated on the conversion of L-Serine to D-Serine, which is metabolized to an intermediate product that’s subsequently oxidized and reacts with a probe to type a secure fluorophore (Ex/Em = 539/587 nm). Samples could also be divided and assayed concurrently for quantification of each D-Serine and whole DL-Serine. The assay is just not affected by physiological concentrations of different amino acids, is high-throughput adaptable and may detect lower than 1 μM Serine

DL-Serine Standard Curve
DL-Serine Customary Curve
Cat # +Dimension Okay743-100
Dimension 100 assays
Equipment Abstract • Detection method- Fluorescence (Ex/Em 535/587 nm)
• Software- Measurement of Serine in organic samples reminiscent of serum, plasma, CSF, and many others.
Detection Technique Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Mammalian
Purposes Estimation of Serine in numerous organic samples
Options & Advantages • Easy, speedy & handy assay
• Restrict of detection: 1 µM
• Very particular, doesn’t work together with different normal and non-standard amino acids
Equipment Parts • Serine Assay Buffer
• Probe Answer
• Serine Racemase Enzyme Combine
• D-Serine Enzyme Combine
• Developer Enzyme Combine
• Pattern Cleanup Combine
• D-Serine Customary
Storage Circumstances -20°C
Delivery Circumstances Gel Pack
USAGE For Analysis Use Solely! Not For Use in People.

 

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