Biovision Rnase-R

RNase R from BioVision


• For digestion of whole RNA, longer incubations of 2-Three hours are sometimes required.
• If degradation is inefficient, use a barely larger incubation temperature (40-45°C) and complement further enzyme partway (e.g. 0.5 μl after 1 hour) via the process. The upper temperature is especially helpful for degrading extremely structured linear RNAs, reminiscent of rRNAs. Don’t exceed 45°C or incubate over 3
hours, as this will result in non-enzymatic RNA degradation.

• RNase R displays low exercise on tRNA, rRNA and different extremely structured RNAs, for which the three’ finish is double stranded with a brief 3’ overhang. These RNA species can stall the enzyme and lead to enormously lowered exercise. If inefficient degradation is noticed, it’s endorsed to both upscale the digestion, use extra RNase R,
or take away rRNA from whole RNA extracts previous to digestion.

• Take into account that round RNAs signify a small proportion of whole RNA (sometimes 0.1%-0.01%), subsequently RNase R remedy will almost certainly lead to low ranges of RNA (picogram-range), probably undetectable by most strategies. For that reason, a beginning quantity of a minimum of 10 µg of whole RNA is advisable for many downstream functions.

• Whereas the enzyme will be warmth inactivated the process is just not advisable since excessive warmth can result in RNA injury. Phenol-chloroform precipitation can be utilized as a substitute. For NGS, strong part reversible immobilization (SPRI) bead cleanup is advisable.

• Magnesium at concentrations of 0.1-1.Zero mM is required for optimum exercise. If ETA is
current, compensate by including MgCl2 to 1.Zero Mm ultimate focus.

DESCRIPTION: RNase R is an E. coli exoribonuclease which displays 3’-to-5’ exonuclease exercise, effectively digesting practically all linear RNA species. This enzyme doesn’t digest round, lariat, or double stranded RNA with quick 3’ overhangs (lower than sevennucleotides). As such, this enzyme is ideally suited to the examine of lariat RNA produced by conventional splicing, in addition to circRNAs which come up via back-splicing. By eradicating linear
RNAs from mobile or RNA extracts, RNase R enormously facilitates the identification of round species via RNA-sequencing. This allows researchers to probe the panorama of splicing occasions with better depth.


Biovision rnase-r
Biovision rnase-r

• Enriching circRNAs in organic samples
• Identification of intronic lariat sequences
• Identification of exonic circRNAs
• Finding out various splicing
• Manufacturing of synthetic round RNAs

EZYME UNIT DEFINITION: One unit is outlined as the quantity of RNase R that converts 1
µg of poly(A) into acid soluble nucleotides in 10 minutes at 37°C. ENZYME STORAGE BUFFER: 50 mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol 10X RNase R REACTION BUFFER COMPONENTS: 200 mM Tris-HCl, 1 M KCl, 1 mM, MgCl2, pH 7.5.

STORAGE CONDITIONS: Retailer at -20°C.

Keep away from repeated freeze-thaw cycles of all elements to retain most efficiency. All elements
are steady for one yr from the date of delivery when saved and dealt with correctly.


Cat # +Measurement M1228-500
Measurement 500 U
Highlights RNase R is an E. coli exoribonuclease which displays 3’-to-5’ exonuclease exercise, effectively digesting practically all linear RNA species.
Storage Situations -20°C
Transport Situations Gel Pack
USAGE For Analysis Use Solely! Not For Use in People.


• RNase A (Cat# M1227-25)
• RNaseOFF ribonuclease Inhibitor (Cat# M1238-4000)
• DNase, E.coli DNA Ligase (Cat# M1217-100)
• Hyperlink-FAST 5 Minutes DNA Ligation Package (Cat# Okay902-50)
• New T4 DNA Ligase (Cat# M1247-200)
• T4 DNA Ligase (5 u/μl) (Cat# 9101-250)
• T4 RNA Ligase 1 (ssRNA Ligase) (Cat# M1218-100)
• T4 RNA Ligase 2 (dsRNA Ligase) (Cat# M1219-100)


RNase R

MBS4156446-500Units 500Units
EUR 330

RNase R

MBS4156446-5x500Units 5x500Units
EUR 1245

RNase R (20 U/μl)

RNR-E5146 2KU
EUR 716.9
Description: RNase R (20 U/μl) is expressed from Escherichia coli K12 E. coli.

RNase H (RNase-free)

K1093-1000 1000U
EUR 42
Description: RNase H

RNase H (RNase-free)

K1093-500 500U
EUR 24
Description: RNase H

RNase H (RNase-free)

K1093-5000 5000U
EUR 120
Description: RNase H


E16FXP0211R-200 200 μl
EUR 13.33
Description: Available in various conjugation types.


E16FXP0211R-400 400 μl
EUR 20
Description: Available in various conjugation types.


E16FXP0211R-800 800 μl
EUR 26.67
Description: Available in various conjugation types.


TRI-A40S1 100UL
EUR 37.8

Rnase (90-105)

MBS8247015-10mg 10mg
EUR 715

Rnase (90-105)

MBS8247015-1mg 1mg
EUR 260

Rnase (90-105)

MBS8247015-5mg 5mg
EUR 510

Rnase (90-105)

MBS8247015-5x10mg 5x10mg
EUR 3140

RNase A

abx098139-1ml 1 ml
EUR 260.4

PI / RNAse

abx290029-200tests 200 tests
EUR 326.4

RNase A

MB087-100MG 1 unit
EUR 34.02
Description: RNase A

RNase A

MB087-1G 1 unit
EUR 284.22
Description: RNase A

RNase A

MB087-500MG 1 unit
EUR 153.8
Description: RNase A

RNase A

RM7705-100MG 1 unit
EUR 136.11
Description: RNase A

RNase A

RM7705-1G 1 unit
EUR 1102.26
Description: RNase A

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