Biovision Rnase-R

RNase R from BioVision


• For digestion of whole RNA, longer incubations of 2-Three hours are sometimes required.
• If degradation is inefficient, use a barely larger incubation temperature (40-45°C) and complement further enzyme partway (e.g. 0.5 μl after 1 hour) via the process. The upper temperature is especially helpful for degrading extremely structured linear RNAs, reminiscent of rRNAs. Don’t exceed 45°C or incubate over 3
hours, as this will result in non-enzymatic RNA degradation.

• RNase R displays low exercise on tRNA, rRNA and different extremely structured RNAs, for which the three’ finish is double stranded with a brief 3’ overhang. These RNA species can stall the enzyme and lead to enormously lowered exercise. If inefficient degradation is noticed, it’s endorsed to both upscale the digestion, use extra RNase R,
or take away rRNA from whole RNA extracts previous to digestion.

• Take into account that round RNAs signify a small proportion of whole RNA (sometimes 0.1%-0.01%), subsequently RNase R remedy will almost certainly lead to low ranges of RNA (picogram-range), probably undetectable by most strategies. For that reason, a beginning quantity of a minimum of 10 µg of whole RNA is advisable for many downstream functions.

• Whereas the enzyme will be warmth inactivated the process is just not advisable since excessive warmth can result in RNA injury. Phenol-chloroform precipitation can be utilized as a substitute. For NGS, strong part reversible immobilization (SPRI) bead cleanup is advisable.

• Magnesium at concentrations of 0.1-1.Zero mM is required for optimum exercise. If ETA is
current, compensate by including MgCl2 to 1.Zero Mm ultimate focus.

DESCRIPTION: RNase R is an E. coli exoribonuclease which displays 3’-to-5’ exonuclease exercise, effectively digesting practically all linear RNA species. This enzyme doesn’t digest round, lariat, or double stranded RNA with quick 3’ overhangs (lower than sevennucleotides). As such, this enzyme is ideally suited to the examine of lariat RNA produced by conventional splicing, in addition to circRNAs which come up via back-splicing. By eradicating linear
RNAs from mobile or RNA extracts, RNase R enormously facilitates the identification of round species via RNA-sequencing. This allows researchers to probe the panorama of splicing occasions with better depth.


Biovision rnase-r
Biovision rnase-r

• Enriching circRNAs in organic samples
• Identification of intronic lariat sequences
• Identification of exonic circRNAs
• Finding out various splicing
• Manufacturing of synthetic round RNAs

EZYME UNIT DEFINITION: One unit is outlined as the quantity of RNase R that converts 1
µg of poly(A) into acid soluble nucleotides in 10 minutes at 37°C. ENZYME STORAGE BUFFER: 50 mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol 10X RNase R REACTION BUFFER COMPONENTS: 200 mM Tris-HCl, 1 M KCl, 1 mM, MgCl2, pH 7.5.

STORAGE CONDITIONS: Retailer at -20°C.

Keep away from repeated freeze-thaw cycles of all elements to retain most efficiency. All elements
are steady for one yr from the date of delivery when saved and dealt with correctly.


Cat # +Measurement M1228-500
Measurement 500 U
Highlights RNase R is an E. coli exoribonuclease which displays 3’-to-5’ exonuclease exercise, effectively digesting practically all linear RNA species.
Storage Situations -20°C
Transport Situations Gel Pack
USAGE For Analysis Use Solely! Not For Use in People.


• RNase A (Cat# M1227-25)
• RNaseOFF ribonuclease Inhibitor (Cat# M1238-4000)
• DNase, E.coli DNA Ligase (Cat# M1217-100)
• Hyperlink-FAST 5 Minutes DNA Ligation Package (Cat# Okay902-50)
• New T4 DNA Ligase (Cat# M1247-200)
• T4 DNA Ligase (5 u/μl) (Cat# 9101-250)
• T4 RNA Ligase 1 (ssRNA Ligase) (Cat# M1218-100)
• T4 RNA Ligase 2 (dsRNA Ligase) (Cat# M1219-100)


PI / RNAse

abx290029-200tests 200 tests
EUR 326.4


PI/RNASE 200 test
EUR 208.2

RNase A

M1227-25 each
EUR 294

RNase A

RN103-001 300mg
EUR 3138

RNase A

RB0473 500mg
EUR 219.73

RNase FREE, 250ml Bottle Removes RNase & DNAse

10-172 1 Bottle/Unit
EUR 269

Rnase (90–105)

5-01885 4 x 1mg Ask for price

Rnase A, 250mg

R9001-250mg each
EUR 337.2

RNase FREE, 1 Liter Bottle Removes RNase & DNAse

10-173 1 Bottle/Unit
EUR 337

RNase FREE, 4 Liter Bottle Removes RNase & DNAse

10-456 1 Bottle/Unit
EUR 403

RNase FREE, 475ml Spray Bottle Removes RNase & DNAse

10-228 1 Spray Bottle/Unit
EUR 300

Rnase A, 1g

R9001-1g each
EUR 986.4

RNase A (MBG)

409-050 50 mg
EUR 87.6

RNase A (MBG)

409-250 250 mg
EUR 231.6

RNase L Antibody / Rnasel

RQ6435 100 ug
EUR 356.15
Description: RNase L is an enzyme that in humans is encoded by the RNASEL gene. This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele.

RNase III, E.coli

M1226-100 each
EUR 268.8

RNase L Peptide

46-735P 0.1 mg
EUR 405.6
Description: ABCE1 / RNase L Peptide

RNase T2 Antibody

abx237324-100ug 100 ug
EUR 610.8

RNase 1 Antibody

  • EUR 444.00
  • EUR 727.20
  • EUR 360.00
  • 100 ul
  • 200 ul
  • 50 ul

RNase 13 Antibody

  • EUR 444.00
  • EUR 727.20
  • EUR 360.00
  • 100 ul
  • 200 ul
  • 50 ul

RNAse H2A Peptide

4979P 0.05 mg
EUR 197.7
Description: (IN) RNAse H2A peptide

Leave a Comment

Your email address will not be published.