Key choices and particulars
- Sensitivity: 10 pg/ml
- Differ: 78 pg/ml – 5000 pg/ml
- Sample type: Cell custom supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
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Product determine
Human BAFF-R ELISA Gear -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Indicate SD CV% 1 16 147pg/ml 10.29 = 7% 2 16 683pg/ml 49.85 = 7.3% 3 16 2750pg/ml 206.25 = 7.5% Inter-assay Sample n Indicate SD CV% 1 24 156pg/ml 13.88 = 8.9% 2 24 700pg/ml 62.3 = 8.9% 3 24 2531pg/ml 227.05 = 8.9% -
Sample type
Cell custom supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Differ
78 pg/ml – 5000 pg/ml -
Assay time
3h 30m -
Assay size
A lot of steps commonplace assay -
Species reactivity
Reacts with: Human
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Product overview
The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) gear (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell custom supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA gear relies on commonplace sandwich enzyme-linked immune-sorbent assay experience. A monoclonal antibody from mouse specific for BAFF-R has been pre-coated onto 96-well plates. Necessities (Expression system for regular: NSO; Immunogen sequence: S7-A71) and check out samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF-R is added subsequently after which adopted by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Difficult is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualise HRP enzymatic response. TMB is catalyzed by HRP to produce a blue shade product that turned yellow after together with acidic stop reply. The density of yellow is proportional to the Human BAFF-R amount of sample captured in plate.
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Notes
BAFF-R (additionally known as Tumor necrosis challenge receptor superfamily member 13C or B-cell-activating challenge receptor) is a protein that in folks is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating challenge (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell inhabitants. The protein encoded by this gene is a receptor for BAFF and is a form III transmembrane protein containing a single extracellular phenylalanine-rich space. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
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Platform
Pre-coated microplate (12 x Eight correctly strips)
Human BAFF (BLyS) Receptor, (Recombinant) |
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| 22060255-1 | Bio-WORLD | 5 µg | EUR 190.94 |
BAFF-R, Human |
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| HY-P7130 | MedChemExpress | 10ug | EUR 183.6 |
BAFF (B-cell Activating Factor) |
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| RC214-15 | Bio Basic | 5ug | EUR 125.26 |
Human BAFF-R(CD268) |
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| 90103-A | BPS Bioscience | 10 µg | EUR 130 |
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Description: Recombinant human BAFF-R (B cell-activating factor from the TNF family), also known as CD268, is a disulfide-linked monomeric protein consisting of 76 a.a. and migrates as an approximately 9 kDa protein under reducing and non-reducing conditions. Optimized DNA sequence encoding human BAFF-R extracellular domain was expressed in E. coli. |
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Human BAFF-R(CD268) |
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| 90103-B | BPS Bioscience | 50 µg | EUR 205 |
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Description: Recombinant human BAFF-R (B cell-activating factor from the TNF family), also known as CD268, is a disulfide-linked monomeric protein consisting of 76 a.a. and migrates as an approximately 9 kDa protein under reducing and non-reducing conditions. Optimized DNA sequence encoding human BAFF-R extracellular domain was expressed in E. coli. |
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Recombinant Human BAFF-R |
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| MBS7611153-005mg | MyBiosource | 0.05mg | EUR 345 |
Recombinant Human BAFF-R |
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| MBS7611153-02mg | MyBiosource | 0.2mg | EUR 635 |
Recombinant Human BAFF-R |
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| MBS7611153-1mg | MyBiosource | 1mg | EUR 1800 |
Recombinant Human BAFF-R |
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| MBS7611153-5x1mg | MyBiosource | 5x1mg | EUR 6955 |
BAFF-R(human):Fc(human) |
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| BC-323 | Kamiya Biomedical Company | 50 ug | EUR 667 |
[Cys0]-BAFF-R (159-183) (Mouse) / [Cys0]-BAFF-R (151-175) (Human) |
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| 030-51 | PHOENIX PEPTIDE | 100 μg | EUR 177.12 |
Human BAFF-R (CD268) Recombinant |
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| MBS553135-001mg | MyBiosource | 0.01mg | EUR 165 |
Human BAFF-R (CD268) Recombinant |
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| MBS553135-005mg | MyBiosource | 0.05mg | EUR 245 |
[Cys0]-BAFF-R (159-183) (Mouse) / [Cys0]-BAFF-R (151-175) (Human) - Antibody |
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| H-030-51 | PHOENIX PEPTIDE | 50 μl | EUR 604.8 |
Recombinant Human BAFF-R (TNFRSF13C) |
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| MBS4159631-005mg | MyBiosource | 0.05mg | EUR 255 |
Recombinant Human BAFF-R (TNFRSF13C) |
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| MBS4159631-01mg | MyBiosource | 0.1mg | EUR 360 |
Recombinant Human BAFF-R (TNFRSF13C) |
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| MBS4159631-1mg | MyBiosource | 1mg | EUR 1335 |
Recombinant Human BAFF-R (TNFRSF13C) |
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| MBS4159631-5x1mg | MyBiosource | 5x1mg | EUR 5770 |
Recombinant Human BAFF-R (TNFRSF13C) |
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| Z100015 | ABM | 50 µg | EUR 105 |
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Description: The B cell-activating factor from the TNF family (BAFF), is emerging as an important regulator of B cell and T cell responses. BAFF was originally identified as a factor responsible for B cell survival and maturation. BAFF binds to several receptors. These include transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), BAFF-R (BR3), and B cell maturation Ag (BCMA). BAFF-R appears to be particularly important for the regulation of B cell survival and maturation in the spleen, because A/WySnJ mice expressing a defective BAFF-R have disrupted B cell maturation, similar to that seen in BAFF-deficient mice. |
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Recombinant Human BAFF-R (TNFRSF13C) |
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| Z100017 | ABM | 100 µg | EUR 215 |
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Description: The B cell-activating factor from the TNF family (BAFF), is emerging as an important regulator of B cell and T cell responses. BAFF was originally identified as a factor responsible for B cell survival and maturation. BAFF binds to several receptors. These include transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), BAFF-R (BR3), and B cell maturation Ag (BCMA). BAFF-R appears to be particularly important for the regulation of B cell survival and maturation in the spleen, because A/WySnJ mice expressing a defective BAFF-R have disrupted B cell maturation, similar to that seen in BAFF-deficient mice. |
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Recombinant Human BAFF-R (TNFRSF13C) |
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| Z100019 | ABM | 1.0 mg | EUR 1060 |
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Description: The B cell-activating factor from the TNF family (BAFF), is emerging as an important regulator of B cell and T cell responses. BAFF was originally identified as a factor responsible for B cell survival and maturation. BAFF binds to several receptors. These include transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), BAFF-R (BR3), and B cell maturation Ag (BCMA). BAFF-R appears to be particularly important for the regulation of B cell survival and maturation in the spleen, because A/WySnJ mice expressing a defective BAFF-R have disrupted B cell maturation, similar to that seen in BAFF-deficient mice. |
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BAFF-R (human):Fc (human), (recombinant) |
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| MBS566224-005mg | MyBiosource | 0.05mg | EUR 540 |
BAFF-R (human):Fc (human), (recombinant) |
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| MBS566224-5x005mg | MyBiosource | 5x0.05mg | EUR 2375 |
BAFF Receptor, Recombinant, Human (BAFF-R, BR3, BLyS Receptor 3) |
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| MBS637358-0005mg | MyBiosource | 0.005mg | EUR 340 |
BAFF Receptor, Recombinant, Human (BAFF-R, BR3, BLyS Receptor 3) |
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| MBS637358-002mg | MyBiosource | 0.02mg | EUR 490 |
BAFF Receptor, Recombinant, Human (BAFF-R, BR3, BLyS Receptor 3) |
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| MBS637358-5x002mg | MyBiosource | 5x0.02mg | EUR 2055 |