quee bio Human baff r

qayee bio human baff r

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Key choices and particulars

 

  • Sensitivity: 10 pg/ml
  • Differ: 78 pg/ml – 5000 pg/ml
  • Sample type: Cell custom supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

 

  • Product determine

    Human BAFF-R ELISA Gear

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Indicate SD CV%
    1 16 147pg/ml 10.29 = 7%
    2 16 683pg/ml 49.85 = 7.3%
    3 16 2750pg/ml 206.25 = 7.5%
    Inter-assay
    Sample n Indicate SD CV%
    1 24 156pg/ml 13.88 = 8.9%
    2 24 700pg/ml 62.3 = 8.9%
    3 24 2531pg/ml 227.05 = 8.9%

  • Sample type

    Cell custom supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 10 pg/ml

  • Differ

    78 pg/ml – 5000 pg/ml

  • Assay time

    3h 30m

  • Assay size

    A lot of steps commonplace assay

  • Species reactivity

    Reacts with: Human
bio human baff r
bio human baff r

  • Product overview

    The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) gear (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell custom supernatants, cell lysates, serum and plasma (heparin, EDTA).

    The ELISA gear relies on commonplace sandwich enzyme-linked immune-sorbent assay experience. A monoclonal antibody from mouse specific for BAFF-R has been pre-coated onto 96-well plates. Necessities (Expression system for regular: NSO; Immunogen sequence: S7-A71) and check out samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF-R is added subsequently after which adopted by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Difficult is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualise HRP enzymatic response. TMB is catalyzed by HRP to produce a blue shade product that turned yellow after together with acidic stop reply. The density of yellow is proportional to the Human BAFF-R amount of sample captured in plate.

  • Notes

    BAFF-R (additionally known as Tumor necrosis challenge receptor superfamily member 13C or B-cell-activating challenge receptor)  is a protein that in folks is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating challenge (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell inhabitants. The protein encoded by this gene is a receptor for BAFF and is a form III transmembrane protein containing a single extracellular phenylalanine-rich space. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

  • Platform

    Pre-coated microplate (12 x Eight correctly strips)

 

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Description: A sandwich quantitative ELISA assay kit for detection of Rat B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.

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