quee bio Human baff r

qayee bio human baff r

Key choices and particulars

 

  • Sensitivity: 10 pg/ml
  • Differ: 78 pg/ml – 5000 pg/ml
  • Sample type: Cell custom supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

 

  • Product determine

    Human BAFF-R ELISA Gear
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Indicate SD CV%
    1 16 147pg/ml 10.29 = 7%
    2 16 683pg/ml 49.85 = 7.3%
    3 16 2750pg/ml 206.25 = 7.5%
    Inter-assay
    Sample n Indicate SD CV%
    1 24 156pg/ml 13.88 = 8.9%
    2 24 700pg/ml 62.3 = 8.9%
    3 24 2531pg/ml 227.05 = 8.9%
  • Sample type

    Cell custom supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Differ

    78 pg/ml – 5000 pg/ml
  • Assay time

    3h 30m
  • Assay size

    A lot of steps commonplace assay
  • Species reactivity

    Reacts with: Human
bio human baff r
bio human baff r
  • Product overview

    The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) gear (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell custom supernatants, cell lysates, serum and plasma (heparin, EDTA).

    The ELISA gear relies on commonplace sandwich enzyme-linked immune-sorbent assay experience. A monoclonal antibody from mouse specific for BAFF-R has been pre-coated onto 96-well plates. Necessities (Expression system for regular: NSO; Immunogen sequence: S7-A71) and check out samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF-R is added subsequently after which adopted by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Difficult is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualise HRP enzymatic response. TMB is catalyzed by HRP to produce a blue shade product that turned yellow after together with acidic stop reply. The density of yellow is proportional to the Human BAFF-R amount of sample captured in plate.

  • Notes

    BAFF-R (additionally known as Tumor necrosis challenge receptor superfamily member 13C or B-cell-activating challenge receptor)  is a protein that in folks is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating challenge (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell inhabitants. The protein encoded by this gene is a receptor for BAFF and is a form III transmembrane protein containing a single extracellular phenylalanine-rich space. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

  • Platform

    Pre-coated microplate (12 x Eight correctly strips)

 

BAFF-R Antibody

3578R-100
EUR 379.2

BAFF-R Antibody

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EUR 175.2

BIO-X-ACT Short DNA Polymerase

BIO-21065 500 Units Ask for price

BIO-X-ACT Short Mix, 2x

BIO-25026 500 Reactions Ask for price

BAFF-R Polyclonal Antibody

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EUR 379.2

BAFF-R Blocking Peptide

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EUR 183.6

BAFF-R Recombinant Protein

90-237 50 ug
EUR 600.9
Description: BAFF is mainly produced by innate immune cells such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells. T cells, activated B cells, some malignant B cells and also non-lymphoid cells like astrocytes, synoviocytes and epithelial cells can also produce BAFF. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and together with IL-6, promotes Ig class-switching and plasma cell differentiation . Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of BAFF leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases, such as Sjögren’s syndrome , Rheumatoid Arthritis (RA) , Multiple sclerosis (MS) and Systemic Lupus Erythematosus (SLE) . BAFF is also increased levels in some lymphoid cancers.

Polyclonal BAFF-R Antibody

APR15125G 0.1mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BAFF-R . This antibody is tested and proven to work in the following applications:

Anti-BAFF-R (human) Monoclonal Antibody (11C1)

M02865 100ug
EUR 535.2
Description: Mouse Monoclonal BAFF-R (human) Antibody (11C1). Validated in Flow Cytometry, IHC and tested in Human.

BIO

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BIO

GL6265-1MG 1 mg
EUR 151.2

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GL6265-25MG 25 mg
EUR 536.4

BIO

GL6265-5MG 5 mg
EUR 234

Human B-Cell Activating Factor (BAFF) ELISA Kit

DLR-BAFF-Hu-48T 48T
EUR 597.6
Description: A sandwich quantitative ELISA assay kit for detection of Human B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human B-Cell Activating Factor (BAFF) ELISA Kit

DLR-BAFF-Hu-96T 96T
EUR 776.4
Description: A sandwich quantitative ELISA assay kit for detection of Human B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human B-Cell Activating Factor (BAFF) ELISA Kit

RD-BAFF-Hu-48Tests 48 Tests
EUR 600

Human B-Cell Activating Factor (BAFF) ELISA Kit

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EUR 830.4

Human B-Cell Activating Factor (BAFF) ELISA Kit

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EUR 626.4

Human B-Cell Activating Factor (BAFF) ELISA Kit

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BAFF (B-cell Activating Factor)

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Mouse B-Cell Activating Factor (BAFF) ELISA Kit

DLR-BAFF-Mu-48T 48T
EUR 609.6
Description: A sandwich quantitative ELISA assay kit for detection of Mouse B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse B-Cell Activating Factor (BAFF) ELISA Kit

DLR-BAFF-Mu-96T 96T
EUR 793.2
Description: A sandwich quantitative ELISA assay kit for detection of Mouse B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat B-Cell Activating Factor (BAFF) ELISA Kit

DLR-BAFF-Ra-48T 48T
EUR 633.6
Description: A sandwich quantitative ELISA assay kit for detection of Rat B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Rat B-Cell Activating Factor (BAFF) ELISA Kit

DLR-BAFF-Ra-96T 96T
EUR 828
Description: A sandwich quantitative ELISA assay kit for detection of Rat B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Mouse B-Cell Activating Factor (BAFF) ELISA Kit

RD-BAFF-Mu-48Tests 48 Tests
EUR 613.2

Mouse B-Cell Activating Factor (BAFF) ELISA Kit

RD-BAFF-Mu-96Tests 96 Tests
EUR 850.8

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