Key choices and particulars
- Sensitivity: 10 pg/ml
- Differ: 78 pg/ml – 5000 pg/ml
- Sample type: Cell custom supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
-
Product determine
Human BAFF-R ELISA Gear -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Indicate SD CV% 1 16 147pg/ml 10.29 = 7% 2 16 683pg/ml 49.85 = 7.3% 3 16 2750pg/ml 206.25 = 7.5% Inter-assay Sample n Indicate SD CV% 1 24 156pg/ml 13.88 = 8.9% 2 24 700pg/ml 62.3 = 8.9% 3 24 2531pg/ml 227.05 = 8.9% -
Sample type
Cell custom supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Differ
78 pg/ml – 5000 pg/ml -
Assay time
3h 30m -
Assay size
A lot of steps commonplace assay -
Species reactivity
Reacts with: Human

-
Product overview
The Human BAFF-R Enzyme-Linked Immunosorbent Assay (ELISA) gear (ab213839) is designed for the quantitative measurement of Human TNFRSF13C in cell custom supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA gear relies on commonplace sandwich enzyme-linked immune-sorbent assay experience. A monoclonal antibody from mouse specific for BAFF-R has been pre-coated onto 96-well plates. Necessities (Expression system for regular: NSO; Immunogen sequence: S7-A71) and check out samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF-R is added subsequently after which adopted by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Difficult is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualise HRP enzymatic response. TMB is catalyzed by HRP to produce a blue shade product that turned yellow after together with acidic stop reply. The density of yellow is proportional to the Human BAFF-R amount of sample captured in plate.
-
Notes
BAFF-R (additionally known as Tumor necrosis challenge receptor superfamily member 13C or B-cell-activating challenge receptor) is a protein that in folks is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating challenge (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell inhabitants. The protein encoded by this gene is a receptor for BAFF and is a form III transmembrane protein containing a single extracellular phenylalanine-rich space. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
-
Platform
Pre-coated microplate (12 x Eight correctly strips)
![]() BAFF-R Antibody |
|||
3578R-100 | Biovision | EUR 316 |
![]() BAFF-R Antibody |
|||
3578R-30T | Biovision | EUR 146 |
![]() BIO-X-ACT Short DNA Polymerase |
|||
BIO-21065 | Bioline | 500 Units | Ask for price |
![]() BIO-X-ACT Short Mix, 2x |
|||
BIO-25026 | Bioline | 500 Reactions | Ask for price |
![]() BAFF-R Polyclonal Antibody |
|||
3578-100 | Biovision | EUR 316 |
![]() BAFF-R Blocking Peptide |
|||
3578RBP-50 | Biovision | EUR 153 |
![]() Polyclonal BAFF-R Antibody |
|||
APR15125G | Leading Biology | 0.1mg | EUR 484 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BAFF-R . This antibody is tested and proven to work in the following applications: |
![]() Anti-BAFF-R (human) Monoclonal Antibody (11C1) |
|||
M02865 | BosterBio | 100ug | EUR 446 |
Description: Mouse Monoclonal BAFF-R (human) Antibody (11C1). Validated in Flow Cytometry, IHC and tested in Human. |
![]() BIO |
|||
1673-1 | Biovision | EUR 158 |
![]() BIO |
|||
GL6265-25MG | Glentham Life Sciences | 25 mg | EUR 447 |
![]() BIO |
|||
GL6265-1MG | Glentham Life Sciences | 1 mg | EUR 126 |
![]() BIO |
|||
GL6265-5MG | Glentham Life Sciences | 5 mg | EUR 195 |
![]() Human B-Cell Activating Factor (BAFF) ELISA Kit |
|||
DLR-BAFF-Hu-48T | DL Develop | 48T | EUR 498 |
Description: A sandwich quantitative ELISA assay kit for detection of Human B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
![]() Human B-Cell Activating Factor (BAFF) ELISA Kit |
|||
DLR-BAFF-Hu-96T | DL Develop | 96T | EUR 647 |
Description: A sandwich quantitative ELISA assay kit for detection of Human B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
![]() Human B-Cell Activating Factor (BAFF) ELISA Kit |
|||
RD-BAFF-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 500 |
![]() Human B-Cell Activating Factor (BAFF) ELISA Kit |
|||
RD-BAFF-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 692 |
![]() Human B-Cell Activating Factor (BAFF) ELISA Kit |
|||
RDR-BAFF-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 522 |
![]() Human B-Cell Activating Factor (BAFF) ELISA Kit |
|||
RDR-BAFF-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 724 |
![]() Mouse B-Cell Activating Factor (BAFF) ELISA Kit |
|||
DLR-BAFF-Mu-48T | DL Develop | 48T | EUR 508 |
Description: A sandwich quantitative ELISA assay kit for detection of Mouse B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
![]() Mouse B-Cell Activating Factor (BAFF) ELISA Kit |
|||
DLR-BAFF-Mu-96T | DL Develop | 96T | EUR 661 |
Description: A sandwich quantitative ELISA assay kit for detection of Mouse B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates or other biological fluids. |
![]() Rat B-Cell Activating Factor (BAFF) ELISA Kit |
|||
DLR-BAFF-Ra-48T | DL Develop | 48T | EUR 528 |
Description: A sandwich quantitative ELISA assay kit for detection of Rat B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
![]() Rat B-Cell Activating Factor (BAFF) ELISA Kit |
|||
DLR-BAFF-Ra-96T | DL Develop | 96T | EUR 690 |
Description: A sandwich quantitative ELISA assay kit for detection of Rat B-Cell Activating Factor (BAFF) in samples from serum, plasma, tissue homogenates, cell lysates or other biological fluids. |
![]() Mouse B-Cell Activating Factor (BAFF) ELISA Kit |
|||
RD-BAFF-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 511 |
![]() Mouse B-Cell Activating Factor (BAFF) ELISA Kit |
|||
RD-BAFF-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 709 |
![]() Rat B-Cell Activating Factor (BAFF) ELISA Kit |
|||
RD-BAFF-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 534 |
![]() Rat B-Cell Activating Factor (BAFF) ELISA Kit |
|||
RD-BAFF-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 742 |