PLANT PRESERVATIVE MIXTURE

Prevalence of various PPM -tolerant endophytic micro organism in papaya, the broad-spectrum microbicide specified to be used in plant tissue cultures, able to surviving covertly in MS-based medium, with implications in contamination administration. Plant Preservative Combination was employed for establishing papaya (Carica papaya) tissue cultures from discipline explants. Evaluating three advisable practices for controlling endogenous microbial contaminants, axillary shoot ideas (1.0-1.5 cm) from cv. Arka Prabhath have been handled with PPM™ 5% for Four h (T1), 50% for 10 min (T2) or 100% for 10 min (T3) and cultured in MS-based papaya institution medium (PEM). By 4-6 weeks, all remedies proved non-rewarding with cultures succumbing both to microbial contamination (80% in T1) or phytotoxicity impact/contamination (90% in T2 and 95% in T3).

One other trial adopting a multi-step floor sterilization remedy (carbendazim-cetrimide-HgCl₂) adopted by culturing in 0.05% PPM-supplemented PEM confirmed 35% apparent bacterial contamination in contrast with 40% in management. Single colonies from pooled bacterial growths have been examined on 0.1% PPM-incorporated nutrient agar (NA) registering 60% isolates as PPM delicate. Twenty PPM-surviving isolates have been chosen and recognized. This confirmed 85% Gram-positive micro organism together with 80% below phylum Firmicutes (55% spore-forming Bacillaceae and 25% Staphylococcaceae) and 5% Actinobacteria, and 15% Gram-negative Proteobacteria.

About 50% isolates remained wholly non-obvious upon culturing on PEM whereas the remaining confirmed gradual progress with many displaying progress enhancement upon host tissue extract supplementation. Culturing the isolates on PPM-supplemented NA indicated 90-95% as tolerating 0.05-0.1% PPM and 65% overriding 0.2% PPM. The isolates, nevertheless, didn’t show apparent progress in PPM-supplemented PEM the place the spore formers survived. The outcomes point out the prevalence of various PPM-tolerant endophytic micro organism in papaya most of which survive covertly in MS-based medium and the necessity for taking this into consideration whereas utilizing PPM for contamination administration.