Histo Clear II

National Diagnostics HISTO-CLEAR II 1 GL

Histo-Clear II

  • Low Hazard
  • Biodegradable
  • Diminished Citrus Odor

Histo-Clear II, like its predecessor Histo-Clear, is a safer histological clearing agent that results in the manufacturing of high-quality tissue slides. Histo-Clear II is unhazardous and utterly biodegradable, thus lowering disposal prices. Histo-Clear II has a tremendously diminished citrus odor in comparison with different citrus-based histological clearing brokers.

Observe: Histo-Clear II isn’t suitable with many manmade mounting media. Histo-Clear II is a long-standing favourite dry mounting procedures and aqueous mounting. Please affirm compatibility together with your mounting media earlier than utilizing Histo-Clear II with in wet-mounting with artificial media.

 

Hazardous Supplies Charge (HazMat) applies if shipped by Air.

Probably the most generally used histological stain, hematoxylin is a pure compound extracted from a species of tree present in Mexico and the West Indies. The extracted compound is oxidized to supply hematein, the energetic staining part of the hematoxylin stain.
The manufacturing of histological staining options is an artwork. Just a few laboratories are able to creating a stain of dependable vibrance. At Nationwide Diagnostics we now have gone one step additional. Our proprietary strategies obtain the best management of the ripening course of, leading to the one commercially obtainable Harris’ Hematoxylin with a full one yr shelf life.

National Diagnostics HISTO-CLEAR
Nationwide Diagnostics HISTO-CLEAR

PROCEDURE
Observe: The next protocol has given good to wonderful outcomes with all kinds of tissue sections. It
could should be optimized for a given tissue, part
thickness, and so forth.

 

1. Dewax and rehydrate slides

a) Immerse slides within the following sequence of baths:
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
l 100% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 50% ethanol – 2 minutes
l Water – 2 minutes

2. Stain
a) Immerse slides in Hematoxylin answer for 10- 20 minutes.
b) Rinse 2 minutes in water or Scott’s Tapwater substitute.
c) Differentiate with 2-5 dips in 70% ethanol containing 0.1% HCl.
d) Immerse for two minutes in Scott’s Tapwater substitute.
e) Stain in 1% aqueous Eosin with 0.1 acetic acid for 10 minutes.

3. Dehydrate, clear and mount
a) Immerse slides within the following sequence of
baths:
l 50% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 100% ethanol – 2 minutes
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
b) Mount coverslip with Histomount.

 

Histo-Clear II

NAT1334 1 US gallon
EUR 151.2

Histo-Clear II

NAT1336 5 US gallons
EUR 457.2

Calci-Clear

NAT1312 EACH
EUR 93.6

Calci-Clear

NAT1314 EACH
EUR 153.6

Calci-Clear

NAT1316 EACH
EUR 463.2

Calci-Clear Rapid

NAT1318 EACH
EUR 90

Calci-Clear Rapid

NAT1320 EACH
EUR 153.6

Calci-Clear Rapid

NAT1322 EACH
EUR 463.2

10X PBS MaxTag Histo (OKRA00043)

OKRA00043 100 ml
EUR 168
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Hematoxylin Counterstain MaxTag Histo (OKRA00050)

OKRA00050 15 ml
EUR 168
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Streptavidin-Peroxidase Conjugate MaxTag Histo (OKRA00036)

OKRA00036 1 each
EUR 339.6
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

PolyMount Mounting Media MaxTag Histo (OKRA00045)

OKRA00045 15 ml
EUR 151.2
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Diogenes Kit

NAT1000 1KIT
EUR 270

Hydrogen Peroxide Assay Kit

NAT1002 1KIT
EUR 308.4

ProtoBlock System

NAT1004 1KIT
EUR 122.4

450ML PBS 10X

NAT1006 450ML
EUR 96

4L PBS 10X

NAT1008 4L
EUR 238.8

ProtoGlow ECL 200ml Kit

NAT1010 EACH
EUR 354

ProtoGlow ECL 500ml Kit

NAT1012 EACH
EUR 627.6

100G Acrylamide

NAT1014 100G
EUR 120

500G Acrylamide

NAT1016 500G
EUR 261.6

1KG Acrylamide

NAT1018 1KG
EUR 412.8

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