Histo-Clear II, like its predecessor Histo-Clear, is a safer histological clearing agent that results in the manufacturing of high-quality tissue slides. Histo-Clear II is unhazardous and utterly biodegradable, thus lowering disposal prices. Histo-Clear II has a tremendously diminished citrus odor in comparison with different citrus-based histological clearing brokers.
Observe: Histo-Clear II isn’t suitable with many manmade mounting media. Histo-Clear II is a long-standing favourite dry mounting procedures and aqueous mounting. Please affirm compatibility together with your mounting media earlier than utilizing Histo-Clear II with in wet-mounting with artificial media.
Hazardous Supplies Charge (HazMat) applies if shipped by Air.
Probably the most generally used histological stain, hematoxylin is a pure compound extracted from a species of tree present in Mexico and the West Indies. The extracted compound is oxidized to supply hematein, the energetic staining part of the hematoxylin stain.
The manufacturing of histological staining options is an artwork. Just a few laboratories are able to creating a stain of dependable vibrance. At Nationwide Diagnostics we now have gone one step additional. Our proprietary strategies obtain the best management of the ripening course of, leading to the one commercially obtainable Harris’ Hematoxylin with a full one yr shelf life.
Nationwide Diagnostics HISTO-CLEAR
PROCEDURE
Observe: The next protocol has given good to wonderful outcomes with all kinds of tissue sections. It
could should be optimized for a given tissue, part
thickness, and so forth.
1. Dewax and rehydrate slides
a) Immerse slides within the following sequence of baths:
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
l 100% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 50% ethanol – 2 minutes
l Water – 2 minutes
2. Stain
a) Immerse slides in Hematoxylin answer for 10- 20 minutes.
b) Rinse 2 minutes in water or Scott’s Tapwater substitute.
c) Differentiate with 2-5 dips in 70% ethanol containing 0.1% HCl.
d) Immerse for two minutes in Scott’s Tapwater substitute.
e) Stain in 1% aqueous Eosin with 0.1 acetic acid for 10 minutes.
3. Dehydrate, clear and mount
a) Immerse slides within the following sequence of
baths:
l 50% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 100% ethanol – 2 minutes
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
b) Mount coverslip with Histomount.
