Histo-Clear II
- Low Hazard
- Biodegradable
- Diminished Citrus Odor
Histo-Clear II, like its predecessor Histo-Clear, is a safer histological clearing agent that results in the manufacturing of high-quality tissue slides. Histo-Clear II is unhazardous and utterly biodegradable, thus lowering disposal prices. Histo-Clear II has a tremendously diminished citrus odor in comparison with different citrus-based histological clearing brokers.
Observe: Histo-Clear II isn’t suitable with many manmade mounting media. Histo-Clear II is a long-standing favourite dry mounting procedures and aqueous mounting. Please affirm compatibility together with your mounting media earlier than utilizing Histo-Clear II with in wet-mounting with artificial media.
Hazardous Supplies Charge (HazMat) applies if shipped by Air.
Probably the most generally used histological stain, hematoxylin is a pure compound extracted from a species of tree present in Mexico and the West Indies. The extracted compound is oxidized to supply hematein, the energetic staining part of the hematoxylin stain.
The manufacturing of histological staining options is an artwork. Just a few laboratories are able to creating a stain of dependable vibrance. At Nationwide Diagnostics we now have gone one step additional. Our proprietary strategies obtain the best management of the ripening course of, leading to the one commercially obtainable Harris’ Hematoxylin with a full one yr shelf life.
PROCEDURE
Observe: The next protocol has given good to wonderful outcomes with all kinds of tissue sections. It
could should be optimized for a given tissue, part
thickness, and so forth.
1. Dewax and rehydrate slides
a) Immerse slides within the following sequence of baths:
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
l 100% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 50% ethanol – 2 minutes
l Water – 2 minutes
2. Stain
a) Immerse slides in Hematoxylin answer for 10- 20 minutes.
b) Rinse 2 minutes in water or Scott’s Tapwater substitute.
c) Differentiate with 2-5 dips in 70% ethanol containing 0.1% HCl.
d) Immerse for two minutes in Scott’s Tapwater substitute.
e) Stain in 1% aqueous Eosin with 0.1 acetic acid for 10 minutes.
3. Dehydrate, clear and mount
a) Immerse slides within the following sequence of
baths:
l 50% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 100% ethanol – 2 minutes
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
b) Mount coverslip with Histomount.
 Histo-Clear II |
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| NAT1334 | National Diagnostics | 1 US gallon | EUR 126 |
Histo-Clear II |
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| NAT1336 | National Diagnostics | 5 US gallons | EUR 381 |
 Calci-Clear |
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| NAT1312 | National Diagnostics | EACH | EUR 78 |
Calci-Clear |
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| NAT1314 | National Diagnostics | EACH | EUR 128 |
Calci-Clear |
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| NAT1316 | National Diagnostics | EACH | EUR 386 |
 Calci-Clear Rapid |
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| NAT1318 | National Diagnostics | EACH | EUR 75 |
Calci-Clear Rapid |
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| NAT1320 | National Diagnostics | EACH | EUR 128 |
Calci-Clear Rapid |
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| NAT1322 | National Diagnostics | EACH | EUR 386 |
) 10X PBS MaxTag Histo (OKRA00043) |
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| OKRA00043 | Aviva Systems Biology | 100 ml | EUR 140 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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) Hematoxylin Counterstain MaxTag Histo (OKRA00050) |
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| OKRA00050 | Aviva Systems Biology | 15 ml | EUR 140 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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) EZ-10 RNA Column and collection tube (clear tube, clear ring, clear collection) |
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| SD5008 | Bio Basic | 100, 100UNIT | EUR 75.88 |
 384 WELL CLEAR RIGI PLATE PCR MICROPLATE. CLEAR |
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| PCR-384-RGD-C | CORNING | 20/pk | EUR 459 |
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Description: PCR Plates & Tubes; A354 PCR Plate-Axygen |
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) Streptavidin-Peroxidase Conjugate MaxTag Histo (OKRA00036) |
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| OKRA00036 | Aviva Systems Biology | 1 each | EUR 283 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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) PolyMount Mounting Media MaxTag Histo (OKRA00045) |
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| OKRA00045 | Aviva Systems Biology | 15 ml | EUR 126 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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 0.6ML MICROTUBES. CLEAR |
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| MCT-060-C | CORNING | 1000/pk | EUR 177 |
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Description: Micro Tubes; Micro Tubes Push Cap- Axygen |
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 1.7ML MICROTUBES. CLEAR |
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| MCT-175-C | CORNING | 500/pk | EUR 109 |
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Description: Micro Tubes; Micro Tubes Push Cap- Axygen |
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 1000UL CLEAR TIPS |
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| T-1000-C | CORNING | 1000/pk | EUR 81 |
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Description: Non Filter Tips; Pipette Tips - Axygen |
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 200UL CLEAR TIPS |
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| T-200-C | CORNING | 1000/pk | EUR 220 |
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Description: Non Filter Tips; Pipette Tips - Axygen |
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 300UL CLEAR TIPS |
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| T-350-C | CORNING | 1000/pk | EUR 133 |
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Description: Non Filter Tips; Pipette Tips - Axygen |
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 Diogenes Kit |
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| NAT1000 | National Diagnostics | 1KIT | EUR 225 |
 Hydrogen Peroxide Assay Kit |
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| NAT1002 | National Diagnostics | 1KIT | EUR 257 |
 ProtoBlock System |
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| NAT1004 | National Diagnostics | 1KIT | EUR 102 |