Lipid Peroxidation (MDA) Assay Kit

Lipid Peroxidation (MDA + HNE) Assay Package deal

Lipid peroxidation is a extensively recognized occasion of oxidative hurt in cell membranes, lipoproteins, and totally different lipid-containing buildings. Peroxidative modification of unsaturated phospholipids, glycolipids, and ldl ldl cholesterol can occur in a number of reactions. They’re typically triggered by i) free radical species equivalent to oxyl radicals, peroxyl radicals, and hydroxyl radicals derived from iron-mediated low cost of hydrogen peroxide or ii) non-radical species equivalent to singlet oxygen, ozone, and peroxynitrite generated by the response of superoxide with nitric oxide.

Malondialdehyde (MDA) and 4-hydroxyalkenals are very important toxic byproducts of lipid peroxidation. So, the measurement of the portions of such aldehydes corresponds to an index of lipid peroxidation in vitro and in vivo. 4-Hydroxynonenal (4-HNE) is a critical product of the peroxidative decomposition of ω-6 polyunsaturated fatty acids (PUFA). It possesses cytotoxic, hepatotoxic, mutagenic, and genotoxic properties. Furthermore, elevated ranges of HNE had been current in plasma and different organs beneath oxidative stress circumstances. The reality is, MDA is in numerous conditions in all probability essentially the most ample specific individual aldehyde ensuing from lipid peroxidation. In vitro MDA can alter proteins, DNA, RNA, and loads of totally different biomolecules.

Bioquochem’s LPO assay gear measures MDA and HNE concentrations as an index of lipid peroxidation. Firstly, acid-catalyzed assault on the 3-position of the indole ring initiates the reactions between indoles and aldehydes (MDA and HNE). Due to this, this response gives a diindolylalkane (chromophore) with most absorbance inside the space of 580-620 nm.

In our assay an indol (Reagent A) reacts quickly with MDA and HNE in acidic medium, yielding a chromophore (C) with a extreme molar extinction coefficient at its maximal absorption wavelength of 586 nm.

lipid-peroxidation-assay-kit
lipid-peroxidation-assay-kit

 

  • Product overview

    Lipid Peroxidation (MDA) Assay Package deal (Colorimetric/Fluorometric) (ab118970) provides a helpful software program for delicate detection of malondialdehyde (MDA).

    Throughout the lipid peroxidation assay protocol, the MDA inside the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct may very well be merely quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA ranges as little as 1 nmol/successfully colorimetrically and 0.1 nmol/successfully fluorometrically.

    The MDA assay may also be refered to as a TBARS assay.

    Lipid peroxidation assay protocol summary:
    – add TBA reply to samples and necessities, incubate at 95ºC for 60 min, cool in ice bathtub for 10 min
    – change to wells of microplate
    – analyze with microplate reader
    For larger sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet sooner than analysis.

    Chinese language language protocol on the market. See protocols half below.

    For an alternate MDA assay, with out the heating steps required inside the TBARS assay, try MDA assay ab233471.

     

     

    Lipid Peroxidation biovision
    Lipid Peroxidation biovision
  • Notes

    Lipid peroxidation refers again to the oxidative degradation of lipids. On this course of free radicals take electrons from the lipids (usually in cell membranes), resulting in cell hurt. Quantification of lipid peroxidation is essential to guage oxidative stress. Lipid peroxidation varieties reactive aldehydes equivalent to malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as pure bi-products. Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are generally used as markers of lipid peroxidation, and to assay for oxidative hurt / oxidative stress.

    Related merchandise

    Evaluation the oxidative stress marker and assay info, or the entire metabolism assay info to review additional assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and as well as how one can assay metabolic function in dwell cells using your plate reader.

    Moreover see the favored 4-HNE Assay Package deal ab238538 in its place marker of lipid peroxidation and oxidative stress.


    How totally different researchers have used Lipid Peroxidation Assay Package deal ab118970

    The MDA/TBARs assay gear has been utilized in publications in a variety of sample types, along with:
    – Human: serum1, hippocampal principal cell extracts2, A375 cultured cell lysates3, plasma and platelet samples4
    – Mouse: neuronal cell lysates5, coronary coronary heart tissue extract6, plasma7, cell extracts8
    – Rat: hippocampal tissue extracts9, cardiomyocyte extracts of cultured cells10, lung lysates11
    – Pig: serum12

    References: 1 – Shen J et al. 2018, 2 – Wang Q et al. 2019, 3 – Luo M et al. 2018,  4 – Mustafa AG et al. 2018, 5 – Murphy Okay et al. 2018, 6 – Guan F et al. 2019, 7 – Costa CRC et al. 2018, 8 – Eleftheriadis T et al. 2019, 9 – Malekiyan et al. 2019, 10 – Zhou Z et al. 2018, 11 – Li L et al. 2018, 12 – Lee SE and Kang KS 2019

  • Platform

    Microplate reader

Lipid Peroxidation Colorimetric Assay Kit

K454-100
EUR 652.8

U-74389G (Lipid peroxidation blocker)

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EUR 208.8
Description: The substance U-74389G is a lipid peroxidation blocker. It is synthetically produced and has a purity of >99%. The pure substance is white solid which is soluble in 25 mg/ml DMSO, and in 20 mg/ml in ethanol; Insoluble in water.

U-74389G (Lipid peroxidation blocker)

SIH-205-500MG 500 mg
EUR 698.4
Description: The substance U-74389G is a lipid peroxidation blocker. It is synthetically produced and has a purity of >99%. The pure substance is white solid which is soluble in 25 mg/ml DMSO, and in 20 mg/ml in ethanol; Insoluble in water.

Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit

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Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit

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Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit

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Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit

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Myeloperoxidase (MPO) Peroxidation Activity Assay Kit

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OxiSelect Myeloperoxidase Peroxidation Activity Assay Kit (Fluorometric)

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Description: Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity. The OxiSelect Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, fluorometric assay for measuring the myeloperoxidase peroxidation activity within a sample.

Lipid A Lipopolysaccharide (LPS Lipid A / Endotoxin) Antibody

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Lipid A Antibody

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Lipid Extraction & Polar/Neutral Lipid Separation Combo Kit (Chloroform Free)

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Description: This kit combines a chloroform free organic lipid extraction with further separation of polar and neutral lipids. The lipid extraction first extracts lipids from a crude lipid source to a chloroform free upper organic phase using a proprietary alcohol. After addition of a proprietary organic compound, the mixture is centrifuged to separate the phases. The recovered organic phase containing lipids is dried and then polar and neutral lipids are separated by adding a proprietary secondary alcohol and organic compound. The layers are separated, dried, and resuspended for further analysis.

Ceraplex Lipid Concentrate(1000X)

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Ceraplex Lipid Concentrate(1000X)

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Lipid Droplet Isolation Kit

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Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer.

Lipid Quantitation Kit (Colorimetric)

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Description: Cell Biolabs? Lipid Quantification Kit measures the lipid content (unsaturated fatty acids only) of samples using the sulfo-phospho-vanillin method, resulting in a simple colorimetric readout amenable to multi-well plate detection.

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