Epigentek

Epigentek Chromoplasts Chromatin Extraction Kit

Background Information
Chromatin immunoprecipitaton (ChIP) provides an advantageous gadget for locating out protein-DNA interaction. With ChIP, the experimenter can resolve if a selected protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a selected gene promoter space beneath quite a few conditions might be achieved via a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Significantly, the ChIP methodology with explicit antibodies straight in direction of quite a few transcriptional parts is broadly demanded.

Product Overview

The ChromaFlash Chromatin Extraction Package deal is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a simple and quick format. Chromatin prepared by this bundle could be utilized in various chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Package deal. The isolated chromatin may even be utilized in numerous chromatin-related features equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

  • Terribly fast course of: your total course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
  • Helpful and versatile: the bundle is suitable for getting ready every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
  • Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.
epigentek chromoplasts chromatin
Epigentek Chromoplasts Chromatin

 

Principle & Course of
The ChromaFlash Chromatin Extraction Package deal accommodates all reagents required for ending up worthwhile chromatin extraction straight from mammalian cells or tissues. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.

Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, modern and frozen tissues, and so forth. The amount of cells and tissues for each preparation might be 1 x 105 to 5 x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 106 cells or 50 to 200 mg tissues.  The yield of chromatin is roughly 4 ug per 106  cells or per 50 mg tissues.

Background Information
Chromatin immunoprecipitation (ChIP) provides an advantageous gadget for locating out protein-DNA interaction. With ChIP, the experimenter can resolve if a selected protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a selected gene promoter space beneath quite a few conditions might be achieved via a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Significantly, a ChIP methodology with explicit antibodies straight in direction of quite a few transcriptional parts is broadly in demand.

 

Principle & Course of
The ChromaFlash Plant Chromatin Extraction Package deal accommodates all the reagents required for ending up a worthwhile chromatin extraction straight from vegetation. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.

ChromaFlash Chromatin Isolation & Shearing Kit 

P-2023
  • EUR 324.48
  • EUR 189.20
  • 100 Preperations
  • 100 Preparations

Chromatin Phosphorylation Sampler Kit

E051006 5x50ug
EUR 700
Description: Available in various conjugation types.

EpiQuik Chromatin Accessibility Assay Kit 

P-1047
  • EUR 910.86
  • EUR 611.60
  • 48 Assays
  • 48 Reactions

miRNA Extraction Kit

K1456-50 50 Preps
EUR 661.2

gPURE EXTRACTION KIT

IB47430 3ML
EUR 30.42
Description: (FOR CULTURED CELLS)

gPURE EXTRACTION KIT

IB47431 100ML
EUR 196.06
Description: (FOR CULTURED CELLS)

gPURE EXTRACTION KIT

IB47432 1000ML
EUR 1358.49
Description: (FOR CULTURED CELLS)

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit 

P-2002
  • EUR 1084.86
  • EUR 312.40
  • EUR 500.50
  • EUR 738.10
  • 96 Reactions
  • 24 Reactions
  • 48 Reactions
  • 96 Reactions

ExoDNA? Extraction Kit

K1242-25 each
EUR 705.6

ExoDNA? Extraction Kit

K1242-50 each
EUR 1057.2

Chromatin protein Cren7 Antibody (HRP)

20-abx109204
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Chromatin protein Cren7 Antibody (FITC)

20-abx107787
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Up RNA Extraction Kit

20-abx098087
  • EUR 309.60
  • EUR 126.00
  • 100 ml
  • 3 ml

Chromatin Modifying Protein 2A Protein

20-abx261989
  • EUR 5388.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Chromatin Modifying Protein 2B Protein

20-abx262025
  • EUR 5388.00
  • EUR 393.60
  • EUR 276.00
  • 1 mg
  • 20 ug
  • 5 ug

Chromatin Modifying Protein 4A Protein

20-abx263203
  • EUR 276.00
  • EUR 1779.60
  • EUR 393.60
  • 1 µg
  • 50 ug
  • 5 ug

Chromatin Modifying Protein 1A Protein

20-abx263326
  • EUR 1930.80
  • EUR 393.60
  • EUR 276.00
  • 100 ug
  • 10 ug
  • 2 µg

Chromatin Protein Cren7 (creN7) Antibody

20-abx109437
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

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