Fast amplification: The amplification fee of the polymerase is 6kb/min. 1kb fragment could be amplified inside 25min.
Lengthy fragment amplification: For plasmid, λ DNA and different straightforward templates, the polymerase can successfully amplify > 20kb. For genome, the polymerase can successfully amplify > 8kb. And for cDNA, the polymerase can successfully amplify > 8kb.
Excessive specificity: With scorching begin expertise, the polymerase is 100% inactive under 50°C, and might solely be restored by heating at 95°C for 5min.
Excessive tolerance to impurities: Samples of entire blood, serum, cultured cells and urine could be immediately amplified with out prior DNA purification.
What’s Direct PCR?
Direct PCR (dPCR) is a technique of DNA amplification immediately from an animal or plant tissue pattern with out performing DNA isolation and purification steps. This system enormously reduces experimental time, and value in genotyping and high-volume tasks. It additionally offers a greater possibility when confronted with the challenges of amplifying a really small amount of pattern the place the purification step may probably result in pattern loss.
In some ways, dPCR works like typical PCR. Each strategies depend upon template DNA, primers and a grasp combine or PCR reagents, and each use a thermal cycler for amplification. The first distinction between dPCR and traditional PCR is the tailor-made buffers utilized in dPCR. The modified components of dPCR allow sturdy amplification regardless of the presence of PCR inhibitors usually present in crude samples.
ALS SARS-CoV-2 RT-PCR 4G has been developed and produced totally at ALS in Portugal and is designed primarily for export. This new equipment is ready to detect all recognized SARS-CoV-2 variants, together with British, Brazilian and South African variants, with out cross-reacting with different recognized associated viruses or frequent respiratory pathogens. The selection of the genomic areas used as targets and the sequences of the primers and hydrolysis probes had been based mostly on WHO, CDC and DGS suggestions.
The ALS SARS-CoV-2 RT-PCR 4G equipment is an in vitro diagnostic medical machine (CE-IVD) that makes use of real-time PCR nucleic acid amplification expertise for the detection of SARS-CoV-2 in scientific samples from the higher respiratory tract of people who might or might not be suspected of getting COVID-19.
The equipment makes use of the RT-PCR method, combining amplification of sure areas of the viral genome with its detection by particular hydrolysis probes. The SARS-CoV-2 genome goal areas are extremely conserved and correspond to 2 particular genes: ORF polyprotein (ORF1ab gene) and nucleocapsid phosphoprotein (N gene). The equipment may detect the gene that codifies the structural protein of the envelope (gene E) particular to the Sarbecovirus subgenus.
Direct PCR Reagent
Unit Definition
One unit is outlined as the quantity of enzyme required to catalyze the incorporation of 10 nmol dNTP into an acid insoluble substance inside 30 min at 74°C.
Description: Intact Genomics FastAmpTM Plant Direct PCR kit is an easy and robust method to amplify DNA fragments from plant tissues with high specificity and high sensitivity without the need of complicated DNA purification steps. The advanced formulation of this kit also allows fast PCR cycling conditions without compromising PCR sensitivity, specificity and yield. The FastAmpTM Plant Direct PCR kit is an ideal and powerful tool for high-throughput genotyping, DNA amplification, and plant genome analysis.Highlights:Direct PCR- no need to purify DNASpecially engineered Taq DNA polymerase with highest sensitivity and specificityExtremely short PCR protocol timesMaster mix format with premixed gel loading dye to reduce cross-contamination and sample handlingerrors5x magic enhancer for high GC containing DNA amplificationProduct Includes:FastAmp™ plant direct PCR master mix (2x)Dilution bufferControl primer mix (25 µM each)5x magic enhancerNuclease- free water
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.
