corticosterone

Corticosterone ELISA kit

Product overview

Corticosterone ELISA  equipment is a aggressive Enzyme-Linked Immunosorbent Assay designed for the quantitative measurement of Corticosterone ranges in plasma, serum, urine, milk, saliva and cell tradition supernatant.

A Corticosterone particular antibody has been precoated onto 96-well plates and blocked. Requirements or take a look at samples are added to the wells and subsequently biotinylated Corticosterone is added after which adopted by washing with wash buffer. Avidin-Biotin-Peroxidase Complicated is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualise HRP enzymatic response. TMB is catalyzed by HRP to provide a blue shade product that adjustments into yellow after including acidic cease resolution. The density of yellow coloration is inversely proportional to the quantity of Corticosterone captured in plate.

Key options and particulars

  • Sensitivity: 0.Three ng/ml
  • Vary: 0.391 ng/ml – 100 ng/ml
  • Pattern kind: Cell tradition supernatant, Milk, Plasma, Saliva, Serum, Urine
  • Detection technique: Colorimetric
  • Assay kind: Aggressive
  • Reacts with: Mouse, Rat, Human, Baboon

 

corticosterone
corticosterone

Product Particulars

Sensitivity: 27.Zero pg/ml (vary 32 – 20,000 pg/ml)
Assay Time: Three hours
Functions: ELISA, Colorimetric detection
Utility Notes: For the quantitative willpower of Corticosterone in tradition supernatants, feces, plasma, serum, and saliva from any species. Cited pattern sorts embrace cell lysate, urine and entire blood.
Wavelength: 405 nm
Species reactivity: Species impartial
Crossreactivity: Corticosterone (100%), Deoxycorticosterone (21.3%), Desoxycorticosterone (21.0%), Progesterone (0.46%), Testosterone (0.31%), Tetrahydrocorticosterone (0.28%), Aldosterone (0.18%), Cortisol (0.046%) and <0.03%: Pregnenolone, Estradiol, Cortisone, 11-dehydrocorticosterone acetate
Transport: Blue Ice Not Frozen
Lengthy Time period Storage: +4°C
Contents: DxS IgG Microtiter plate, Assay buffer 15 focus, Steroid displacement reagent, Conjugate, Antibody, Wash buffer focus, Normal, pNpp Substrate, Cease resolution
Scientific Background: Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to the stimulation of the adrenal cortex by adrenocorticotropic hormone (ACTH) and is the precursor of aldosterone. Corticosterone manufacturing has been proven to extend with stress, with elevated corticosterone ranges being related to impairment of long run reminiscence retrieval and following burn damage. Along with stress ranges, corticosterone is believed to play a decisive function in sleep-wake patterns.
Technical Data/Product Notes: Cited samples:
Hormone ELISAs: Cited Samples
Wildlife Endocrinology: Cited Pattern Varieties
Protocol: Urged Protocol for Small Volumes of Serum/Plasma Samples

  1. Let all options come to room temp earlier than use.
  2. Aliquot 10µl of every pattern into microfuge tube.
  3. Make 1ml 1:100 Steroid Displacement Reagent (SDR) resolution (instantly earlier than use) in deionized water or PBS (not assay buffer)
  4. Add 10µl 1:100 SDR to every pattern tube.
  5. Vortex, let stand >5 minutes earlier than diluting with EIA buffer.
  6. Add 380µl EIA assay buffer to every plasma tube, vortex.

Last dilution is ~1:40 (This ultimate dilution might not be applicable for each pattern; thus, optimum dilution should be decided by every finish consumer for his or her experimental pattern group.)

Regulatory Standing: RUO – Analysis Use Solely

 

Human Estrone-3-Glucuronide (E1G)Enzyme Immunoassay ELISA Kit

QY-E05423 96T
EUR 433.2

Mouse Angiotensin I Converting Enzyme Chemiluminescent Immunoassay Kit

IMSACEKTC each
EUR 545
Description: Mouse Angiotensin I Converting Enzyme Chemiluminescent Immunoassay Kit

Human Angiotensin I Converting Enzyme Chemiluminescent Immunoassay Kit

IHUACEKTC each
EUR 545
Description: Human Angiotensin I Converting Enzyme Chemiluminescent Immunoassay Kit

Human Angiotensin I Converting Enzyme 2 Chemiluminescent Immunoassay Kit

IHUACE2KTC each
EUR 545
Description: Human Angiotensin I Converting Enzyme 2 Chemiluminescent Immunoassay Kit

11,12-DHET Immunoassay Kit

DH4 1 Kit
EUR 368.4

(1-Kit )11,12-EET/DHET Immunoassay Kit

DH5 1 Kit
EUR 368.4

CaspSELECT Caspase 3 Immunoassay Kit

55R-1338 100 assays
EUR 1226.4
Description: Assay Kit for detection of Capase 3 activity in the research laboratory

Mouse Monokine induced by interferon-gammaChemiluminescent Immunoassay Kit Chemiluminescent Immunoassay Kit

IMSCXCL9KTC each
EUR 545
Description: Mouse Monokine induced by interferon-gammaChemiluminescent Immunoassay Kit Chemiluminescent Immunoassay Kit

Mouse Macrophage Inflammatory Protein 1 BetaChemiluminescent Immunoassay Kit Chemiluminescent Immunoassay Kit

IMSMIP1BKTC each
EUR 545
Description: Mouse Macrophage Inflammatory Protein 1 BetaChemiluminescent Immunoassay Kit Chemiluminescent Immunoassay Kit

cGMP Direct Immunoassay Kit (Colorimetric)

K372-100 each
EUR 614.4

cAMP Direct Immunoassay Kit (Colorimetric)

K2044-100 100 assays
EUR 634.8

cGMP Direct Immunoassay Kit (Colorimetric)

K2066-100 100 assays
EUR 634.8

cAMP Direct Immunoassay Kit (Colorimetric)

K371-100 each
EUR 614.4

Human Mothers Against Decapentaplegic Homolog 4 Chemiluminescent Immunoassay Kit Chemiluminescent Immunoassay Kit

IHUSMAD4KTC each
EUR 545
Description: Human Mothers Against Decapentaplegic Homolog 4 Chemiluminescent Immunoassay Kit Chemiluminescent Immunoassay Kit

Immunoassay Stabilizer

85R-103 1 liter
EUR 577.2
Description: Stabilizing buffer for use in immunoassays

Rat Renin Chemiluminescent Immunoassay Kit

IRTRENKTC each
EUR 545
Description: Rat Renin Chemiluminescent Immunoassay Kit

QuickTiter Adenovirus Titer Immunoassay Kit

VPK-109 100 assays
EUR 846
Description: Accurate measurement of adenovirus titer is critical for gene delivery. Traditional plaque-forming unit (PFU) assays are long and suffer from high inter-assay variability. The QuickTiter Adenovirus Titer Immunoassay Kit provide a quick, complete system to functionally titer virus infectivity. The assay recognizes all 41 serotypes of adenovirus, and can be used with any adenovirus system that can amplify in HEK 293 cells.

Rat Leptin Chemiluminescent Immunoassay Kit

IRTLEPKTC each
EUR 545
Description: Rat Leptin Chemiluminescent Immunoassay Kit

Rat Desmin Chemiluminescent Immunoassay Kit

IRTDESKTC each
EUR 545
Description: Rat Desmin Chemiluminescent Immunoassay Kit

 

Leave a Comment

Your email address will not be published.