BrdU Cell Proliferation

BrdU Cell Proliferation ELISA Kit

 

Product Description

The BrdU Cell Proliferation Assay Bundle detects 5-bromo-2’-deoxyuridine (BrdU) built-in into cellular DNA all through cell proliferation using an anti-BrdU antibody. When cells are cultured with labeling medium that comes with BrdU, this pyrimidine analog is built-in as an alternative of thymidine into the newly synthesized DNA of proliferating cells.

After eradicating labeling medium, cells are mounted and the DNA is denatured with our fixing/denaturing reply. Then a BrdU mouse mAb is added to detect the built-in BrdU (The denaturing of DNA is essential to boost the accessibility of the built-in BrdU to the detection antibody). Anti-mouse IgG, HRP-linked antibody is then used to acknowledge the sure detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU built-in into cells, which is a direct indication of cell proliferation.

Product Consists of Quantity (with Rely) Reply Color
BrdU 1 x 150 µl
Fixing/denaturing Reply 2 x 25 ml
BrdU Mouse Detection mAb 1 x 500 µl Inexperienced
Anti-mouse IgG, HRP-Linked Antibody 1 x 500 µl Pink
Detection Antibody Diluent 1 x 50 ml Inexperienced
HRP Diluent 1 x 50 ml Pink
20X Wash Buffer 1 x 50 ml
TMB Substrate 7004 1 x 50 ml
STOP Reply 7002 2 x 25 ml

Background

Halogenated nucleotides such as a result of the pyrimidine analog bromodeoxyuridine (BrdU) are useful for labeling nascent DNA in residing cells and tissues. BrdU turns into built-in into replicating DNA as an alternative of thymidine and subsequent immunodetection of BrdU using specific monoclonal antibodies permits labeling of cells in S a part of the cell cycle. After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb will be utilized to detect BrdU built-in into single stranded DNA.

BrdU Cell Proliferation ELISA Kit (colorimetric)
BrdU Cell Proliferation ELISA Package (colorimetric)

Key choices and particulars

  • Sensitivity: 40 cells/successfully
  • Sample sort: Adherent cells, Suspension cells
  • Detection approach: Colorimetric
  • Assay sort: Sandwich (qualitative)

5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog. It should get built-in into the newly synthesized DNA of proliferating cells as an alternative of thymidine. Biovision’s BrdU Cell Proliferation Assay Bundle detects built-in BrdU using a mouse anti-BrdU antibody. An anti-mouse HRP-linked secondary antibody is used to detect the anti-BrdU antibody sure to BrdU, which is adopted by addition of TMB (a HRP substrate). The extent of color enchancment is proportional to the quantity of BrdU built-in into the cells and will be utilized straight as an indicator of cell proliferation. As compared with completely different cell proliferation assays, this package deal detects solely the proliferating cells and by no means the seeded cells. This extraordinarily delicate, non-radioactive package deal detects as a lot much less as 50-100 proliferating cells.

 

Brdu Cell
Brdu Cell

METABOLIC PROLIFERATION ASSAYS

Assays that measure metabolic train are applicable for analyzing proliferation, viability, and cytotoxicity. The low cost of tetrazolium salts just like MTT, XTT, and WST-1 to colored formazan compounds or the bioreduction of resazurin occurs solely in metabolically energetic cells. Actively proliferating cells improve their metabolic train, whereas cells uncovered to toxins could have decreased train.

MTT Cell Proliferation Assays

MTT (3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide; thiazolyl blue) is a water soluble tetrazolium salt yielding a yellowish reply when prepared in media or salt choices lacking phenol pink. Dissolved MTT is remodeled to an insoluble purple formazan by cleavage of the tetrazolium ring by dehydrogenase enzymes. This water-insoluble formazan can be solubilized using isopropanol or completely different solvents, and the dissolved supplies is measured spectrophotometrically using absorbance as a function of focus of remodeled dye.

XTT Cell Proliferation Assays

In distinction to MTT, the cleavage product of XTT is soluble in water; a solubilization step is subsequently not required. The tetrazolium salt XTT is cleaved to formazan by a flowery cellular mechanism. This bioreduction occurs in viable cells solely, and is expounded to NAD(P)H manufacturing by way of glycolysis. The amount of formazan dye customary straight correlates to the number of metabolically energetic cells inside the custom.

WST-1 Cell Proliferation Assays

The regular tetrazolium salt WST-1 is cleaved to a soluble formazan by a flowery cellular mechanism that occurs totally on the cell ground. This bioreduction is mainly relying on the glycolytic manufacturing of NAD(P)H in viable cells. The amount of formazan dye customary straight correlates to the number of metabolically energetic cells inside the custom.

 

CytoSelect BrdU Competitive ELISA Kit

CBA-5098 96 assays
EUR 543

CytoSelect MTT Cell Proliferation Assay

CBA-252 960 assays
EUR 409
Description: Cell Biolabs? CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation.  The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1).  An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

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Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF594 conjugate, Concentration: 0.1mg/mL

Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody

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Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF594 conjugate, Concentration: 0.1mg/mL

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BNC802888-100 100uL
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Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF680 conjugate, Concentration: 0.1mg/mL

Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody

BNC802888-500 500uL
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Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF680 conjugate, Concentration: 0.1mg/mL

Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody

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Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF680R conjugate, Concentration: 0.1mg/mL

Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody

BNC812888-500 500uL
EUR 545
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF680R conjugate, Concentration: 0.1mg/mL

Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody

BNCAP2888-100 100uL
EUR 233
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody

BNCAP2888-500 500uL
EUR 545
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody

BNC472888-100 100uL
EUR 233
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF647 conjugate, Concentration: 0.1mg/mL

Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody

BNC472888-500 500uL
EUR 545
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF647 conjugate, Concentration: 0.1mg/mL

Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody

BNC432888-100 100uL
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Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF543 conjugate, Concentration: 0.1mg/mL

Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody

BNC432888-500 500uL
EUR 545
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF543 conjugate, Concentration: 0.1mg/mL

Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody

BNC882888-100 100uL
EUR 233
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF488A conjugate, Concentration: 0.1mg/mL

Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody

BNC882888-500 500uL
EUR 545
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF488A conjugate, Concentration: 0.1mg/mL

Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody

BNCH2888-100 100uL
EUR 233
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody

BNCH2888-500 500uL
EUR 545
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody

BNCP2888-250 250uL
EUR 394
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), PerCP conjugate, Concentration: 0.1mg/mL

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BNC042888-100 100uL
EUR 233
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF405S conjugate, Concentration: 0.1mg/mL

Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R) Antibody

BNC042888-500 500uL
EUR 545
Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), CF405S conjugate, Concentration: 0.1mg/mL

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Description: Primary antibody against Bromodeoxyuridine (BrdU) (Proliferation Marker) (BRD2888R), RPE conjugate, Concentration: 0.1mg/mL

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