Concanavalin A from Canavalia ensiformis (Jack bean) has been used for creating zwitterion-immobilized molecularly imprinted polymers (ziMIPs) for protein crystallization. It has been additionally used within the preparation of protein loaded microgels to stop enzymatic degradation of microgels for monitoring by confocal laser scanning microscope (CLSM).
1 g in poly bottle
25, 100, 250 mg in poly bottle
Con A shouldn’t be blood group particular however has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for exercise. Con A dissociates into dimers at pH 5.6 or beneath. Between pH 5.eight and pH 7.0, Con A exists as a tetramer; above pH 7.Zero larger aggregates are shaped. Con A reveals mitogenic exercise which relies on its diploma of aggregation. Succinylation ends in an lively dimeric kind which stays a dimer above pH 5.6.
Extremely purified by particular affinity chromatography.
Agglutination exercise: Decided from serial dilutions of a 1 mg/mL answer (this exercise is the bottom conc. to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C). Requires Sort A Human Blood.
Concanavalin A (Con A) is likely one of the most generally used lectins in cell biology. Our Alexa Fluor 488 conjugate of Con A reveals the brilliant, inexperienced fluorescence of the Alexa Fluor® 488 dye (absorption/emission maxima ~495/519 nm). Alexa Fluor 488 Con A selectively binds to a-mannopyranosyl and a-glucopyranosyl residues.
Concanavalin A (Con A) is a extensively used lectin that selectively binds to the glycoproteins, a-mannopyranosyl and a-glucopyranosy. These are generally discovered within the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues.
Stain the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues
Detect glycoconjugates in microscopy and circulation cytometry
Stain glycoproteins in gels
Withstands fixation and permeabilization
Alternative of 10 CF dyes from UV to near-infrared
Superior CF dyes are shiny, photostable, and water-soluble
Lectins are additionally versatile probes for detecting glycoconjugates in microscopy and circulation cytometric purposes and for gel staining of glycoproteins. In impartial and alkaline options, Con A exists as a tetramer with a molecular weight of roughly 104 kDa. In acidic options (pH beneath 5.0), Con A exists as a dimer. Con A can be utilized to selectively stain the cell floor of stay cells, and face up to fixation and permeabilization. When cells are mounted and permeabilized earlier than staining, fluorescent lectins stain each cell floor and organelles within the secretory pathway.
Discover the Proper Stain for Your Utility
Con A and different lectins are carbohydrate binding proteins that acknowledge particular sugar moieties on glycoproteins. The presence and distribution of those targets fluctuate between cell sorts and tissues. Consequently, different cell floor stains or different lectin conjugates, Wheat Germ Agglutinin (WGA) Conjugates and PNA Lectin Conjugates, could produce higher floor staining and could also be extra applicable to your cell kind. Lectin conjugates can be utilized to selectively stain the cell floor of stay cells, and face up to fixation and permeabilization. When cells are mounted and permeabilized earlier than staining, fluorescent lectins stain each cell floor and organelles within the secretory pathway.