Background
Cell proliferation shall be detected by numerous methods. Cell proliferation induces an increase inside the train of the mitochondrial dehydrogenases, which cleaves the tetrazolium salt to formazan.
The WST Cell Proliferation Colorimetric Assay Bundle plus provides the very best and most delicate technique for quantification of cell proliferation and viability. Cell proliferation causes the rise inside the amount of formazan dye normal that is straight proportional to the number of residing cells and shall be quantified by measuring the absorbance of the dye decision at 440 nm using microtiter plate reader. The tools could be utilized for the analysis of cell proliferation in response to pharmaceutical compounds, cytotoxic compounds like anticancer medication and many completely different toxic brokers. The assay might also be used for the detection of cell proliferation in response to cytokines, mitogens, progress parts and nutritional vitamins, and so forth. The assay could possibly be very simple and simple, merely add-and-read, requiring no solubilization, no washing and no harvesting steps, and is regular, sooner and further delicate than MTS, MTT or XTT-based assays. Your complete assay shall be carried out in a microtiter plate and correlates properly with the [3H]-thymidine incorporation assay.
| Choices | Fast and helpful Straightforward one-step course of; takes decrease than 4 hours. Makes use of a water-soluble tetrazolium salt WST reagent. The technique is very easy that requires no washing/harvesting/or solubilization steps and is faster and further delicate than MTT/XTT/or MTS-based assays. Your complete assay shall be carried out straight in a 96-well plate. |
| Supply | Gel pack |
| Storage Circumstances | Retailer at -20°C. |
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- 2. Xiangli Zhuang, Bo Wu, et al. “Influence of Xingbi Gel Nasal Drops on Fyn-STAT5 Pathway in Nasal Mucosa Fibroblasts of Guinea Pigs with Allergic Rhinitis.” Evid Based Complement Alternat Med. 2021 Mar 20;2021:6686815. PMID:33824677
- 3. Rui-Qi Wang, Xiao-Ran Prolonged, et al. “Lnc-GAN1 expression is said to good survival and suppresses tumor growth by sponging mir-26a-5p to activate PTEN signaling in non-small cell lung most cancers.” J Exp Clin Most cancers Res. 2021 Jan 6;40(1):9. PMID:33407724
- 4. Peng Zhao, Jianhua Cheng, et al. “LncRNA PCAT6 regulates the event of pituitary adenomas by regulating the miR-139-3p/BRD4 axis.” Most cancers Cell Int. 2021 Jan 6;21(1):14. PMID:33407504
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| K1018-100 | ApexBio | 20x5 ml (10000 tests) | EUR 885 |
Cell Counting Kit-8 (CCK-8) |
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| K1018-30 | ApexBio | 6x5 ml (3000 tests) | EUR 328 |
Cell Counting Kit-8 (CCK-8) |
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| K1018-5 | ApexBio | 1x5 ml (500 tests) | EUR 119 |
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| 20-abx098245 | Abbexa |
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CCK-8 Cell Counting Kit |
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| A311-02 | Vazyme | 1000 rxn | EUR 234 |
Cell Counting Kit-8 (CCK-8) |
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| B34302 | Bimake | 5 mL (500 ractions) | EUR 130 |
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Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading. |
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Cell Counting Kit-8 (CCK-8) |
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| B34304 | Bimake | 25 mL (2500 ractions) | EUR 315 |
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Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading. |
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Cell Counting Kit-8 (CCK-8) |
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Description: Lab Equipment; Cell Counters |
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 CytoSelect BrdU Cell Proliferation ELISA Kit |
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| CBA-251 | Cell Biolabs | 96 assays | EUR 531 |
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Description: The CytoSelect BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody. When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells. Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection). Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU. The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation. |
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